Differential expression patterns of secreted frizzled related protein genes in synovial cells from patients with arthritis

J Rheumatol. 2002 Nov;29(11):2266-70.

Abstract

Objective: To detect expression of the secreted frizzled related protein (sFRP) gene in synovial cells from patients with arthritis.

Methods: Expression of sFRP-1, 2, 3, 4, and 5 genes was detected in synovial cells from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis. To identify synovial cell populations expressing sFRP-1, 3, and 4 genes, expression was compared in macrophage-rich populations and fibroblast-like cell-rich populations by RT-PCR. Levels of expression of these genes were also studied in activated peripheral blood mononuclear cells (PBMC) and activated skin fibroblasts.

Results: Expression of the sFRP-1, 3, and 4 genes was observed in both RA and OA synovial cells. sFRP-1 and 4 genes were expressed predominantly in fibroblast-like cell-rich populations, and the sFRP-3 gene was expressed predominantly in macrophage-rich populations. Levels of expression of sFRP-3 and 4 genes were elevated in activated PBMC and activated skin fibroblasts.

Conclusion: Our findings suggest that sFRP-1, 3, and 4 may play different roles in the pathogenesis of synovitis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis / genetics*
  • Arthritis / metabolism*
  • Arthritis / physiopathology
  • Arthritis, Rheumatoid / genetics
  • Arthritis, Rheumatoid / metabolism
  • Arthritis, Rheumatoid / physiopathology
  • Cells, Cultured
  • Fibroblasts / metabolism
  • Gene Expression Regulation / physiology
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Macrophages / metabolism
  • Osteoarthritis / genetics
  • Osteoarthritis / metabolism
  • Osteoarthritis / physiopathology
  • Proteins / genetics*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism
  • Signal Transduction / genetics
  • Synovial Membrane / metabolism*
  • Synovial Membrane / physiopathology
  • Wnt Proteins
  • Zebrafish Proteins*

Substances

  • Intracellular Signaling Peptides and Proteins
  • Proteins
  • Proto-Oncogene Proteins
  • WD repeat containing planar cell polarity effector
  • Wnt Proteins
  • Zebrafish Proteins
  • frizzled related protein-3