Abstract
Actin-related proteins share significant homology with conventional actins and are classified into subfamilies based on the similarity of their sequences and functions. The Arp4 subfamily of Arps is localized in the nucleus, and a mammalian isoform, ArpNbeta (also known as BAF53), is a component of the chromatin remodeling and histone acetyltransferase complexes. Another isoform identified in humans, ArpNalpha has scarcely been characterized yet. We identified mouse ArpNalpha, and showed that ArpNalpha is more similar between humans and mice than ArpNbeta. No difference was observed between ArpNalpha and beta in subcellular localization and interaction with BRM, which is an ATPase subunit of mammalian SWI/SNF chromatin remodeling complex. However, ArpNalpha was expressed exclusively in the brain and its expression was induced during neural differentiation of P19 mouse embryonic carcinoma cells. ArpNalpha is the first brain-specific component of a chromatin remodeling complex to be identified, suggesting that ArpNalpha has conserved and important roles in the differentiation of neural cells through regulation of chromatin structure.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / genetics
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Adenosine Triphosphatases / metabolism
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Amino Acid Sequence
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Animals
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Brain / metabolism*
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Cell Cycle Proteins / metabolism*
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Cell Differentiation
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Chromatin / metabolism
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Chromosomal Proteins, Non-Histone / biosynthesis*
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / physiology*
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DNA-Binding Proteins
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Drosophila Proteins
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Gene Expression Regulation
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Humans
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Macromolecular Substances
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Mice
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Molecular Sequence Data
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Neurons / metabolism
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Nuclear Proteins / biosynthesis*
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Nuclear Proteins / genetics
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Nuclear Proteins / physiology*
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Organ Specificity
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RNA, Messenger / biosynthesis
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Sequence Homology, Amino Acid
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Tissue Distribution
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Trans-Activators / metabolism*
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Transcription Factors / metabolism
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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ACTL6B protein, human
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Actins
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Actl6b protein, mouse
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Cell Cycle Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Drosophila Proteins
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Macromolecular Substances
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Nuclear Proteins
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RNA, Messenger
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Trans-Activators
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Transcription Factors
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brm protein, Drosophila
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Adenosine Triphosphatases