Mutagenic transgene insertion into a region of high gene density and multiple linkage disruptions on mouse chromosome 11

Cytogenet Genome Res. 2002;97(1-2):100-5. doi: 10.1159/000064062.

Abstract

We have characterized a 185-kb contig surrounding a transgene on distal mouse chromosome 11, the insertion of which has caused a recessive phenotype with skeletal malformations. By cDNA selection and sequencing we have found six genes (Lasp1, Rpl23, Mllt6, Pip5k2b, Psmb3, Zfp144), one truncated gene (Mel13), and one pseudogene (Rps15-ps) within this region. The murine Mllt6 gene is new, it was identified by its high homology (90% identity) with the human homologue MLLT6. Psmb3 and Pip5k2b had not yet been assigned to mouse chromosomes. A comparison with the corresponding region on human chromosome 17q12 revealed several small-scale rearrangements during evolutionary divergence within this cluster of densely packed genes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Base Sequence
  • Chromosomes, Mammalian / genetics*
  • Collagen / genetics
  • Collagen Type I*
  • Collagen Type I, alpha 1 Chain
  • Cytoskeletal Proteins
  • DNA Transposable Elements
  • DNA, Complementary / genetics
  • DNA-Binding Proteins / genetics
  • Evolution, Molecular
  • Gene Rearrangement
  • Genetic Linkage
  • Homeodomain Proteins / genetics
  • Humans
  • Iron-Sulfur Proteins
  • LIM Domain Proteins
  • Lac Operon
  • Mice
  • Mice, Transgenic
  • Mitochondrial Proteins
  • Molecular Sequence Data
  • Multigene Family
  • Mutagenesis, Insertional
  • Mutation*
  • Neoplasm Proteins / genetics
  • Physical Chromosome Mapping
  • Proteins / genetics
  • Pseudogenes
  • Ribosomal Proteins / genetics
  • Species Specificity

Substances

  • Adaptor Proteins, Signal Transducing
  • Cisd3 protein, mouse
  • Collagen Type I
  • Collagen Type I, alpha 1 Chain
  • Cytoskeletal Proteins
  • DNA Transposable Elements
  • DNA, Complementary
  • DNA-Binding Proteins
  • Homeodomain Proteins
  • Iron-Sulfur Proteins
  • LASP1 protein, human
  • LIM Domain Proteins
  • Lasp1 protein, mouse
  • MLLT6 protein, human
  • Mitochondrial Proteins
  • Mllt6 protein, mouse
  • Neoplasm Proteins
  • Proteins
  • Ribosomal Proteins
  • ribosomal protein S15
  • Collagen

Associated data

  • GENBANK/AY050217
  • GENBANK/AY050218