Objective: To investigate the relationship between tumor necrosis factor A-308 gene polymorphism and susceptibility of severe periodontitis in adults.
Methods: Buccal swabs from 65 adult patients with severe periodontitis and 96 periodontal healthy adult controls were obtained. DNA was extracted from each sample using Chelex-100 method, then PCR-RFLP was performed to determine the TNFA-308 genotype. Data were analyzed by statistics software.
Results: A significant difference of genotype distribution was found between the patients and controls. The percentage of 1/1 type of the patients was much higher (P < 0.001). Risk Analysis showed odds ratio for allele 1 was 4.64.
Conclusion: This study suggested that TNFA-308 allele 1 maybe a marker of susceptibility to severe periodontitis.