Abstract
We show that the glial cell line-derived neurotrophic factor (GDNF) activates the PI3K/Akt-signaling pathway in human neuroblastoma cells that express functional Ret-receptor complexes. Consistent with this finding we show PI3K-dependent Bad-inactivation by binding to 14-3-3 proteins in response to GDNF. Using differential display techniques we detected several cDNA clones differentially expressed after treatment with GDNF or 6-OHDA.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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14-3-3 Proteins
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Gene Expression Regulation, Neoplastic / drug effects*
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Glial Cell Line-Derived Neurotrophic Factor
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Humans
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Hydroxydopamines / pharmacology
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Neoplasm Proteins / genetics
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Nerve Growth Factors / pharmacology*
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Neuroblastoma
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Neuroprotective Agents / pharmacology*
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Phosphatidylinositol 3-Kinases / metabolism
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Polymerase Chain Reaction
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Signal Transduction / drug effects
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Signal Transduction / physiology*
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Tumor Cells, Cultured
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Tyrosine 3-Monooxygenase / metabolism
Substances
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14-3-3 Proteins
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GDNF protein, human
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Glial Cell Line-Derived Neurotrophic Factor
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Hydroxydopamines
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Neoplasm Proteins
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Nerve Growth Factors
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Neuroprotective Agents
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5-hydroxydopamine
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Tyrosine 3-Monooxygenase
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Phosphatidylinositol 3-Kinases