The COS-7 cell in vitro paradigm to study myelin proteolipid protein 1 gene mutations

Methods Mol Biol. 2003:217:263-75. doi: 10.1385/1-59259-330-5:263.

Author

Alexander Gow¹

Affiliation

¹ Center for Molecular Medicine and Genetics, Departments of Pediatrics and Neurology, Wayne State University School of Medicine, Detroit, MI, USA.

PMID: 12491939
DOI: 10.1385/1-59259-330-5:263

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Base Sequence
COS Cells*
Chlorocebus aethiops
Chromosome Mapping
Cloning, Molecular / methods
DNA Primers
Disease Models, Animal
Electrophoresis, Agar Gel / methods
Exons
Humans
Immunohistochemistry / methods
Indicators and Reagents
Membrane Proteins*
Models, Biological
Molecular Sequence Data
Mutation*
Mutation, Missense
Myelin Proteolipid Protein / chemistry
Myelin Proteolipid Protein / genetics*
Pelizaeus-Merzbacher Disease / genetics*
Plasmids
Protein Biosynthesis
Transfection
X Chromosome

Substances

DNA Primers
Indicators and Reagents
Membrane Proteins
Myelin Proteolipid Protein
PLP1 protein, human