Characterization of a new family of proteins that interact with the C-terminal region of the chromatin-remodeling factor CHD-3

FEBS Lett. 2003 Jan 2;533(1-3):14-20. doi: 10.1016/s0014-5793(02)03737-7.

Abstract

The two human proteins Ki-1/57 and CGI-55 have highly similar amino acid sequences but their functions are unknown. We analyzed them by yeast two-hybrid screens and found that they interact with the C-terminal region of the human chromatin-remodeling factor CHD-3 (chromo-helicase-DNA-binding domain protein-3). The interaction of CGI-55 and CHD-3 could be confirmed in vitro and in vivo by co-immunoprecipitations from Sf9 insect cells. Mapping showed that CGI-55 interacts with CHD-3 via two regions at its N- and C-terminals. The CGI-55 and Ki-1/57 mRNAs show highest expression in muscle, colon and kidney. A CGI55-GFP fusion protein was localized in the cytoplasm, nucleus and perinuclear regions of HeLa cells. These data suggest the possibility that CGI-55 and Ki-1/57 might be involved in nuclear functions like the remodeling of chromatin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases / chemistry*
  • Adenosine Triphosphatases / genetics
  • Adenosine Triphosphatases / metabolism*
  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Caenorhabditis elegans Proteins / chemistry
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Chromatin / metabolism
  • DNA Helicases / chemistry*
  • DNA Helicases / genetics
  • DNA Helicases / metabolism*
  • Gene Expression
  • HeLa Cells
  • Humans
  • Hyaluronan Receptors / chemistry*
  • Hyaluronan Receptors / genetics
  • Hyaluronan Receptors / metabolism*
  • In Vitro Techniques
  • Ki-1 Antigen / chemistry*
  • Ki-1 Antigen / genetics
  • Ki-1 Antigen / metabolism*
  • Mi-2 Nucleosome Remodeling and Deacetylase Complex
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • RNA-Binding Proteins / chemistry*
  • RNA-Binding Proteins / genetics
  • RNA-Binding Proteins / metabolism*
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • Sequence Homology, Amino Acid
  • Tissue Distribution

Substances

  • Caenorhabditis elegans Proteins
  • Chromatin
  • Hyaluronan Receptors
  • Ki-1 Antigen
  • RNA, Messenger
  • RNA-Binding Proteins
  • Recombinant Proteins
  • SERBP1 protein, human
  • Mi-2 Nucleosome Remodeling and Deacetylase Complex
  • Adenosine Triphosphatases
  • DNA Helicases
  • CHD3 protein, human

Associated data

  • GENBANK/AF016672
  • GENBANK/AF151813
  • GENBANK/AF241831
  • GENBANK/AL080119