Abstract
ZAK has been implicated in cell cycle arrest regulation through its function on decreasing cyclin E expression. To explore the mechanistic basis for this regulation, the yeast two-hybrid system was used with a novel Krüppel-type C2H2 zinc finger member cloned. This cloned cDNA encodes a novel protein with Krüppel-type zinc fingers designed as ZZaPK (zinc finger and ZAK associated protein with KRAB domain) and is widely expressed. ZZaPK, when it is expressed in cells, is growth promoted and might lead to increasing E2F expression and induce cyclin E/CDK2 activity, which counteracts the ZAK function. The model proposed here is that ZAK might play a role as an upstream signal to suppress the ZZaPK function and decrease E2F expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding Sites
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Cell Cycle / physiology*
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Cell Cycle Proteins / metabolism
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Cell Line
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Cyclin E / metabolism
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DNA-Binding Proteins / metabolism
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E2F Transcription Factors
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Female
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Humans
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Leucine Zippers
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MAP Kinase Kinase Kinases
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Models, Biological
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Molecular Sequence Data
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Placenta / physiology
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Protein Binding
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Protein Kinases / chemistry
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Protein Structure, Tertiary
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Rats
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Retinoblastoma Protein / metabolism
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Tissue Distribution
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Transcription Factors / chemistry
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
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Transcription Factors / metabolism*
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Two-Hybrid System Techniques
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Zinc Fingers*
Substances
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Cell Cycle Proteins
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Cyclin E
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DNA-Binding Proteins
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E2F Transcription Factors
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Recombinant Fusion Proteins
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Retinoblastoma Protein
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Transcription Factors
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ZNF33A protein, human
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Protein Kinases
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MAP Kinase Kinase Kinases
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MAP3K20 protein, human