[Study on the fusion expression of FBXO30: a novel member of F-box protein family]

Hunan Yi Ke Da Xue Xue Bao. 2001 Dec 28;26(6):495-8.
[Article in Chinese]

Abstract

The cDNA sequence of the coding region of FBXO30 (F-box only protein 30), which is a novel member of F-box protein family, was cloned into the mammalian expression vector pEGFP-C2 by non-directional cloning method and introduced into the NIH 3T3 cells by liposome transfection. Observation under the fluorescent microscopy after transfection showed that EGFP (enhanced green fluorescent protein)/FBXO30 was expressed and existed mainly in the cytoplasm. At the same time, the cDNA sequence of the coding region of FBXO30 was cloned into prokaryotic expression vector pGEX-4T-2 by directional cloning strategy. The GST (glutathione S-transferase)/FBXO30 fusion protein was expressed under the induction of IPTG (isopropylthio-beta-D-galactoside) in E. coli. A new band (approximately 65.5 kD) was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting. Purification of the GST-fused FBXO30 was carried out by affinity chromatography with glutathione sepharose 4B. The fusion expression of FBXO30 indicates that: FBXO30 protein is a cytoplasmic soluble protein, and the stable fusion expression of FBXO30 in the prokaryotic expression system can provide a base for the preparation of the specific antibody of FBXO30 and further functional study of FBXO30.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells / metabolism
  • Animals
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics*
  • Drosophila Proteins
  • Escherichia coli / genetics
  • GATA Transcription Factors
  • Glutathione Transferase / genetics
  • Homeodomain Proteins / genetics*
  • Mice
  • Oncogene Proteins, Fusion / biosynthesis*
  • Oncogene Proteins, Fusion / genetics
  • Recombinant Fusion Proteins / biosynthesis*
  • Recombinant Fusion Proteins / genetics
  • Transcription Factors / genetics*
  • Transfection

Substances

  • DNA-Binding Proteins
  • Drosophila Proteins
  • GATA Transcription Factors
  • Homeodomain Proteins
  • Oncogene Proteins, Fusion
  • Recombinant Fusion Proteins
  • Transcription Factors
  • srp protein, Drosophila
  • Glutathione Transferase