Abstract
Actin-related protein (Arp) is found in many chromatin remodeling and histone acetyltransferase complexes. We previously identified ArpN alpha as an isoform of ArpN beta/BAF53, which is included in mammalian SWI/SNF chromatin remodeling complex, and showed that ArpN alpha is a potential component of the complex. Although it has a structure highly similar to ArpN beta/BAF53, ArpN alpha is expressed exclusively in brain and in neural differentiated embryonal carcinoma cells. Since ArpN alpha possesses a region that shows low similarity to ArpN beta/BAF53, we hypothesized that proteins interacting with this region contribute to the ArpN alpha-specific function in brain. Here we showed that ArpN alpha, but not ArpN beta/BAF53, interacts with the transcriptional co-repressor CtBP (C-terminal binding protein). Transactivation by the SWI/SNF complex and glucocorticoid receptor was repressed by the CtBP in the presence of ArpN alpha. These findings suggest that SWI/SNF complex containing ArpN alpha might regulate certain genes involved in brain development and/or its function differently from SWI/SNF complex containing ArpN beta/BAF53.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / chemistry
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Actins / metabolism
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Alcohol Oxidoreductases
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Amino Acid Sequence
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Animals
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Brain / metabolism*
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Brain / physiology
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COS Cells
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Chromosomal Proteins, Non-Histone / chemistry
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation
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Genes, Reporter
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Humans
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Mice
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Models, Molecular
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Molecular Sequence Data
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Nuclear Proteins / chemistry
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism*
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Phosphoproteins / genetics
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Phosphoproteins / metabolism*
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Promoter Regions, Genetic
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Protein Isoforms / chemistry
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Protein Isoforms / genetics
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Protein Isoforms / metabolism*
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Protein Structure, Tertiary
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Repressor Proteins / chemistry
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Sequence Alignment
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Transcriptional Activation
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Two-Hybrid System Techniques
Substances
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ACTL6B protein, human
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Actins
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Chromosomal Proteins, Non-Histone
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DNA-Binding Proteins
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Nuclear Proteins
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Phosphoproteins
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Protein Isoforms
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Repressor Proteins
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Transcription Factors
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Alcohol Oxidoreductases
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C-terminal binding protein