[Study on the Polymorphism of Intron 22 (CA)n Repeat within FVIII Gene in Dai, Yi and Han Populations of Yunnan Province]

Hong Tai; Zijin Dian; Hongmei Ouyang; Zhenyu Li; Haiying Wan; Changgeng Ruan

[Study on the Polymorphism of Intron 22 (CA)n Repeat within FVIII Gene in Dai, Yi and Han Populations of Yunnan Province]

Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2000 Jun;8(2):142-144.

[Article in Chinese]

Authors

Hong Tai¹, Zijin Dian, Hongmei Ouyang, Zhenyu Li, Haiying Wan, Changgeng Ruan

Affiliation

¹ Department of Medical Laboratory Sciences, The First People's Hospital of Yunnan Province, Kunming 650032, China; Jiangsu Institute of Hematology, Thrombosis and Hemostasis Research Unit of the First Affiliated Hospital, Suzhou Medical College, Suzhou 215006, China.

PMID: 12578706

Abstract

This study was designed to investigate the polymorphism of intron 22 (CA)n repeat within FVIII gene in Dai, Yi and Han populations of Yunnan province. PCR, DNA sequencing technique and PAGE were used in this study. The results showed that three different alleles corresponding to 24, 25 and 26 dinucleotides were found at this locus and the allele frequency ranged from 1.20% to 57.7% in Dai and from 16.43% to 63.00% in Yi populations. In Han population, five different alleles with 24, 25, 26, 27 and 28 (CA)s were found, the allele frequency ranged from 8.97% to 32.00%. Heterozygotes of intron 22 (CA) repeat within FVIII gene were not found in the three populations. In conclusion, it was obvious that the locus cannot be acted as DNA genetic marker of FVIII gene in the three populations in Yunnan.