Effect of P-glycoprotein and multidrug resistance associated protein gene expression on Tc-99m MIBI imaging in hepatocellular carcinoma

Chi-Sen Chang; Wen-Tao Huang; Sheng-Shun Yang; Hong-Zen Yeh; Chia-Hung Kao; Gran-Hum Chen

doi:10.1016/s0969-8051(02)00414-6

Effect of P-glycoprotein and multidrug resistance associated protein gene expression on Tc-99m MIBI imaging in hepatocellular carcinoma

Nucl Med Biol. 2003 Feb;30(2):111-7. doi: 10.1016/s0969-8051(02)00414-6.

Authors

Chi-Sen Chang¹, Wen-Tao Huang, Sheng-Shun Yang, Hong-Zen Yeh, Chia-Hung Kao, Gran-Hum Chen

Affiliation

¹ Division of Gastroenterology, Taichung Veterans General Hospital and Department of Internal Medicine, Chung San Medical University, Taichung, Taiwan. changcs@vghtc.gov.tw

PMID: 12623109
DOI: 10.1016/s0969-8051(02)00414-6

Abstract

P-glycoprotein (Pgp) and multidrug resistance-associated protein (MRP) expressions as well as Tc-99m methoxisobutylisonitrile (MIBI) images were assessed in 25 patients hepatocellular carcinoma (HCC). Tc-99m MIBI imaging was performed 10 minutes after intravenous injection of 20 mCi Tc-99m MIBI. Using immunohistochemical staining, 60% of the HCC lesions showed positive for Pgp and 64% showed positive for MRP. In 3 patients with MIBI uptake, immunohistochemical study of tumor tissue showed no Pgp stained cells. Nevertheless, they were all positive for MRP. The result of Tc-99m MIBI imaging is more related to the expression of Pgp than MRP gene. It is possible that other membrane transporters as well as Pgp and MRP are involved in the efflux of Tc-99m MIBI.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

ATP Binding Cassette Transporter, Subfamily B, Member 1 / analysis
ATP Binding Cassette Transporter, Subfamily B, Member 1 / genetics
ATP Binding Cassette Transporter, Subfamily B, Member 1 / metabolism*
Adult
Aged
Carcinoma, Hepatocellular / diagnostic imaging*
Carcinoma, Hepatocellular / metabolism*
Carcinoma, Hepatocellular / pathology
Female
Gene Expression Regulation, Neoplastic / genetics
Genes, MDR
Humans
Liver Neoplasms / diagnostic imaging
Liver Neoplasms / metabolism
Male
Metabolic Clearance Rate
Middle Aged
Multidrug Resistance-Associated Proteins / analysis
Multidrug Resistance-Associated Proteins / genetics
Multidrug Resistance-Associated Proteins / metabolism*
Radionuclide Imaging
Radiopharmaceuticals / metabolism
Radiopharmaceuticals / pharmacokinetics
Technetium Tc 99m Sestamibi / metabolism*
Technetium Tc 99m Sestamibi / pharmacokinetics*

Substances

ATP Binding Cassette Transporter, Subfamily B, Member 1
Multidrug Resistance-Associated Proteins
Radiopharmaceuticals
Technetium Tc 99m Sestamibi