Abstract
An autoantibody against SS-A/Ro52 (Ro52) is most frequently found in the sera of patients with Sjögren's syndrome, systemic lupus erythematosus, and congenital heart block from anti-Ro52 Ab-positive mother. However, the physiological function of the autoantigen SS-A/Ro52 has not yet been elucidated. In this study, we describe the role of Ro52 protein in T cell activation. Overexpression of SS-A/Ro52 in Jurkat T cell resulted in enhanced IL-2 production following CD28 stimulation. Furthermore, transfection of anti-Ro52-specific small RNA duplexes partially blocked the expression of native and overexpressed Ro52 in Jurkat T cell, resulting in decreased IL-2 production via CD28 pathway in these cells. Finally, intracellular localization of Ro52 dramatically changed following CD28 stimulation. Our data reveal a novel function of Ro52 in CD28-mediated pathway, which eventually contributes to cytokine production and expression of the T cell biological programs.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adjuvants, Immunologic / genetics
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Adjuvants, Immunologic / physiology
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Autoantigens / genetics
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Autoantigens / physiology*
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CD28 Antigens / physiology*
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Down-Regulation / genetics
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Down-Regulation / immunology
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Green Fluorescent Proteins
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Humans
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Interleukin-2 / antagonists & inhibitors
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Interleukin-2 / biosynthesis*
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Jurkat Cells
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Luminescent Proteins / genetics
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Lymphocyte Activation
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RNA Interference / immunology
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RNA, Small Cytoplasmic*
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Ribonucleoproteins / antagonists & inhibitors
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Ribonucleoproteins / genetics
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Ribonucleoproteins / physiology*
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Signal Transduction / genetics
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Signal Transduction / immunology
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Subcellular Fractions / immunology
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Subcellular Fractions / metabolism
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T-Lymphocyte Subsets / immunology
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T-Lymphocyte Subsets / metabolism
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Transfection
Substances
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Adjuvants, Immunologic
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Autoantigens
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CD28 Antigens
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Interleukin-2
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Luminescent Proteins
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RNA, Small Cytoplasmic
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RO60 protein, human
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Ribonucleoproteins
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SS-A antigen
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Green Fluorescent Proteins