Abstract
Gene expression in Rat-1 fibroblast cells transformed by Tax from human T-cell leukemia virus type 1 was studied using the reverse transcriptase polymerase chain reaction differential display technique. The analysis revealed eight genes that were upregulated and one gene that was suppressed in Tax-transformed cells. Interestingly, at least four of the upregulated genes were interferon-stimulated genes. Promoter analysis of the 2',5'-oligoadenylate synthetase gene, which was activated in both Tax-transformed Rat-1 cells and primary adult T-cell leukemia cells, demonstrated that Tax indirectly activates its interferon-responsive enhancer element in a nuclear factor-kappaB pathway-dependent manner, indicating a close association of interferon signaling with the transformation by Tax.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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2',5'-Oligoadenylate Synthetase / genetics
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Animals
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Cell Line, Transformed
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Cell Transformation, Viral
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Chemokine CXCL10
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Chemokines, CXC / pharmacology
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Enhancer Elements, Genetic*
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Gene Expression Regulation, Viral
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Gene Products, tax / physiology*
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HeLa Cells
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Human T-lymphotropic virus 1 / physiology*
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Humans
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Interferon-gamma / pharmacology
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Leukemia-Lymphoma, Adult T-Cell / blood
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Lymphocytes / metabolism
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Molecular Sequence Data
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NF-kappa B / metabolism*
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Rats
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction
Substances
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Chemokine CXCL10
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Chemokines, CXC
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Gene Products, tax
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NF-kappa B
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Interferon-gamma
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2',5'-Oligoadenylate Synthetase
Associated data
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GENBANK/AF065438
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GENBANK/AF067793
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GENBANK/AF068268
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GENBANK/AF168795