Abstract
Amitotically activated mitogen-activated protein kinase 1 (MEK1) fragments the pericentriolar Golgi stacks in mammalian cells. We show that activated MEK1 is found on the Golgi apparatus in late prophase. The fragmented and dispersed Golgi membranes in prometaphase and later stages of mitosis do not contain activated MEK1. MEK1-dependent Golgi complex fragmentation is through activation by RAF1 and not MEK1 kinase 1. We propose that a RAF1-dependent activation of MEK1 and its presence on the Golgi apparatus in late prophase is required for Golgi complex fragmentation.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Active Transport, Cell Nucleus / drug effects
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Active Transport, Cell Nucleus / genetics
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Animals
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Aphidicolin / pharmacology
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COS Cells
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Cell Compartmentation / drug effects
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Cell Compartmentation / genetics
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Eukaryotic Cells / metabolism*
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Eukaryotic Cells / ultrastructure
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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HeLa Cells
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Humans
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Intracellular Membranes / drug effects
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Intracellular Membranes / metabolism
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MAP Kinase Kinase 1
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Mice
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Mitogen-Activated Protein Kinase Kinases / genetics
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Mitogen-Activated Protein Kinase Kinases / metabolism*
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Mitosis / genetics*
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Prophase / genetics*
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Protein Serine-Threonine Kinases / genetics
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Protein Serine-Threonine Kinases / metabolism*
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Protein Transport / drug effects
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Protein Transport / genetics
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Proto-Oncogene Proteins c-raf / genetics
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Proto-Oncogene Proteins c-raf / metabolism*
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Rats
Substances
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Aphidicolin
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Protein Serine-Threonine Kinases
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Proto-Oncogene Proteins c-raf
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MAP Kinase Kinase 1
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MAP2K1 protein, human
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Map2k1 protein, mouse
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Mitogen-Activated Protein Kinase Kinases