Orthotopic implantation of a colon cancer xenograft induces high expression of cyclooxygenase-2

Cancer Lett. 2003 Jun 10;195(2):235-41. doi: 10.1016/s0304-3835(02)00108-8.

Abstract

The aim of the present study is to investigate the relation of cyclooxygenase (COX)-2 with liver metastasis. Pieces of human colon cancer xenograft were implanted orthotopically (CI), as well as intraperitoneally, and subcutaneously. Liver metastasis developed most frequently, and the COX-2 expression of both mRNA and protein of the tumors was the most dominant in the CI group. In contrast, the expression of COX-1 or vascular endothelial cell growth factor mRNAs had no significant differences among the groups. The intensity of COX-2 mRNA was negatively correlated with the apoptotic index. In conclusion, COX-2 plays an important role in organ-specific metastasis of the colon cancer.

Publication types

  • Comparative Study

MeSH terms

  • Adenocarcinoma / blood supply
  • Adenocarcinoma / enzymology
  • Adenocarcinoma / pathology
  • Adenocarcinoma / secondary*
  • Animals
  • Apoptosis
  • Cecum
  • Colonic Neoplasms / enzymology
  • Colonic Neoplasms / pathology*
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Endothelial Growth Factors / biosynthesis
  • Endothelial Growth Factors / genetics
  • Enzyme Induction
  • Gene Expression Regulation, Neoplastic*
  • Intercellular Signaling Peptides and Proteins / biosynthesis
  • Intercellular Signaling Peptides and Proteins / genetics
  • Isoenzymes / biosynthesis*
  • Isoenzymes / genetics
  • Liver Neoplasms / blood supply
  • Liver Neoplasms / enzymology
  • Liver Neoplasms / secondary*
  • Lymphokines / biosynthesis
  • Lymphokines / genetics
  • Male
  • Membrane Proteins
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Neoplasm Proteins / biosynthesis*
  • Neoplasm Proteins / genetics
  • Neoplasm Transplantation / methods*
  • Neovascularization, Pathologic
  • Organ Specificity
  • Peritoneal Cavity
  • Prostaglandin-Endoperoxide Synthases / biosynthesis*
  • Prostaglandin-Endoperoxide Synthases / genetics
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • RNA, Neoplasm / biosynthesis
  • RNA, Neoplasm / genetics
  • Skin
  • Specific Pathogen-Free Organisms
  • Transplantation, Heterologous
  • Transplantation, Heterotopic
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors

Substances

  • Endothelial Growth Factors
  • Intercellular Signaling Peptides and Proteins
  • Isoenzymes
  • Lymphokines
  • Membrane Proteins
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Vascular Endothelial Growth Factor A
  • Vascular Endothelial Growth Factors
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • Prostaglandin-Endoperoxide Synthases
  • Ptgs1 protein, mouse