Effect of p53 gene transfection on vascular endothelial growth factor expression in endometrial cancer cells

T Fujisawa; J Watanabe; Y Kamata; M Hamano; H Hata; H Kuramoto

doi:10.1016/s0014-4800(03)00020-0

Effect of p53 gene transfection on vascular endothelial growth factor expression in endometrial cancer cells

Exp Mol Pathol. 2003 Jun;74(3):276-81. doi: 10.1016/s0014-4800(03)00020-0.

Authors

T Fujisawa¹, J Watanabe, Y Kamata, M Hamano, H Hata, H Kuramoto

Affiliation

¹ Department of Clinical Cytology, Graduate School of Medical Sciences, Kitasato University, Sagamihara, Kanagawa, Japan. takeshi@cc.ahs.kitasato-u.ac.jp

PMID: 12782015
DOI: 10.1016/s0014-4800(03)00020-0

Abstract

It has been reported that tumor suppressor gene p53 regulates vascular endothelial growth factor (VEGF) expression, but the relation between them in endometrial carcinoma remains unclear. We investigated VEGF expression in 11 endometrial carcinoma cell lines and the effect of p53 gene transfection on VEGF expression in the p53-mutated endometrial carcinoma cell line, HEC-50B. Immunoblotting for detecting VEGF, p53, and beta-actin was performed. Wild type p53 gene was transfected using the SuperFect method. The mean VEGF value of 0.8 +/- 0.3 (n = 6) in p53 wild-type group was significantly lower than the 1.6 +/- 0.8 (n = 5) that was found in the p53 mutant group (P < 0.05). Levels of VEGF in the culture medium were measured by enzyme immunoassay (EIA). VEGF levels in the p53 gene-transfected HEC-50B cells and the conditioned medium were decreased at 48 h after p53 gene transfection. VEGF expression was downregulated by p53 in endometrial carcinoma cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carcinoma / chemistry
Carcinoma / genetics
Carcinoma / metabolism*
Culture Media, Conditioned / chemistry
Down-Regulation
Endometrial Neoplasms / chemistry
Endometrial Neoplasms / genetics
Endometrial Neoplasms / metabolism*
Endothelial Growth Factors / analysis
Endothelial Growth Factors / metabolism*
Female
Gene Expression Regulation, Neoplastic*
Genes, p53*
Humans
Immunoenzyme Techniques
Immunohistochemistry
Intercellular Signaling Peptides and Proteins / analysis
Intercellular Signaling Peptides and Proteins / metabolism*
Lymphokines / analysis
Lymphokines / metabolism*
Transfection*
Tumor Cells, Cultured
Tumor Suppressor Protein p53 / genetics
Tumor Suppressor Protein p53 / metabolism*
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors

Substances

Culture Media, Conditioned
Endothelial Growth Factors
Intercellular Signaling Peptides and Proteins
Lymphokines
Tumor Suppressor Protein p53
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factors