Abstract
We demonstrate here that Chinese hamster ovary cells stably expressing PRL-3, a M(r) 20000 prenylated protein tyrosine phosphatase, or its relative, PRL-1, exhibit enhanced motility and invasive activity. A catalytically inactive PRL-3 mutant has significantly reduced migration-promoting activity. We observe that PRL-3 is associated with diverse membrane structures involved in cell movement. Furthermore, we show that PRL-3- and -1-expressing cells, but not control cells, induce metastatic tumor formation in mice. Thus, our results deliver the first evidence for a causative role of PRL-3 and -1 in promoting cell motility, invasion activity, and metastasis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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CHO Cells
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Cell Membrane / enzymology
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Cell Membrane / physiology
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Cell Movement / physiology*
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Cricetinae
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Female
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Immediate-Early Proteins / genetics
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Immediate-Early Proteins / metabolism
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Immediate-Early Proteins / physiology*
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Mice
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Mice, Nude
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Neoplasm Invasiveness
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Neoplasm Metastasis
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Neoplasms, Experimental / enzymology*
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Neoplasms, Experimental / pathology*
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Protein Tyrosine Phosphatases / genetics
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Protein Tyrosine Phosphatases / metabolism
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Protein Tyrosine Phosphatases / physiology*
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Proto-Oncogene Proteins c-myc / biosynthesis
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Proto-Oncogene Proteins c-myc / genetics
Substances
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Immediate-Early Proteins
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Proto-Oncogene Proteins c-myc
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Ptp4a3 protein, mouse
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Protein Tyrosine Phosphatases
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Ptp4a1 protein, mouse