Abstract
stx(2) genes from 138 Shiga toxin-producing Escherichia coli (STEC) isolates, of which 127 were of bovine origin (58 serotypes) and 11 of human origin (one serotype; O113:H21), were subtyped. The bovine STEC isolates from Australian cattle carried ehxA and/or eaeA and predominantly possessed stx(2-EDL933) (103 of 127; 81.1%) either in combination with stx(2vhb) (32 of 127; 25.2%) or on its own (52 of 127; 40.4%). Of 22 (90.9%) bovine isolates of serotype O113:H21, a serotype increasingly recovered from patients with hemolytic uremic syndrome (HUS) or hemorrhagic colitis, 20 contained both stx(2-EDL933) and stx(2vhb); 2 isolates contained stx(2vhb) only. Although 7 of 11 (63.6%) human O113:H21 isolates associated with diarrhea possessed stx(2-EDL933), the remaining 4 isolates possessed a combination of stx(2-EDL933) and stx(2vhb). Three of the four were from separate sporadic cases of HUS, and one was from an unknown source.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adhesins, Bacterial / genetics
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Adhesins, Bacterial / metabolism
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Animals
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Carrier Proteins / genetics
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Carrier Proteins / metabolism
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Cattle
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Cattle Diseases / microbiology*
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Escherichia coli / classification*
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Escherichia coli / pathogenicity*
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism
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Hemolysin Proteins / genetics
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Hemolysin Proteins / metabolism
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Humans
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Serotyping
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Shiga Toxin 1 / genetics
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Shiga Toxin 1 / metabolism
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Shiga Toxin 2 / classification*
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Shiga Toxin 2 / genetics
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Shiga Toxin 2 / metabolism
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Virulence Factors / genetics
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Virulence Factors / metabolism
Substances
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Adhesins, Bacterial
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Carrier Proteins
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Escherichia coli Proteins
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Hemolysin Proteins
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Hlya protein, E coli
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Shiga Toxin 1
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Shiga Toxin 2
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Virulence Factors
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eaeA protein, E coli