Chronic myeloid leukemia associated with B-cell chronic lymphocytic leukemia: evidence of two separate clones as shown by combined cell-sorting and fluorescence in situ hybridisation

Leuk Lymphoma. 2003 May;44(5):867-9. doi: 10.1080/1042819031000063435.

Abstract

The simultaneous occurrence of Philadelphia positive chronic myeloid leukemia (Ph+ CML) and B-cell chronic lymphocytic leukemia (B-CLL) is a rare event which raises the possibility that the two malignant clones derive from a common, or distinct, malignant stem cells. In this study, we used combined CD19-based cell-sorting and fluorescence in situ hybridisation (FISH) to investigate whether or not the BCR-ABL fusion gene was present in the malignant B-cells of a patient who presented a Ph+ CML/B-CLL association. The CD19+ cells lacked the BCR-ABL rearrangement whereas all CD19-cells exhibited the fusion gene. This result demonstrates that B-cell transformation occurred in a Ph-B-cell subset.

Publication types

  • Case Reports

MeSH terms

  • Aged
  • Antigens, CD19 / analysis
  • B-Lymphocytes / pathology
  • Cell Transformation, Neoplastic
  • Clone Cells / pathology
  • DNA, Neoplasm / analysis
  • Flow Cytometry
  • Fusion Proteins, bcr-abl / analysis
  • Fusion Proteins, bcr-abl / genetics
  • Humans
  • In Situ Hybridization, Fluorescence
  • Leukemia, Lymphocytic, Chronic, B-Cell / diagnosis
  • Leukemia, Lymphocytic, Chronic, B-Cell / etiology
  • Leukemia, Lymphocytic, Chronic, B-Cell / pathology*
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / diagnosis
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / etiology
  • Leukemia, Myelogenous, Chronic, BCR-ABL Positive / pathology*
  • Male
  • Neoplasms, Multiple Primary / diagnosis
  • Neoplasms, Multiple Primary / etiology
  • Neoplasms, Multiple Primary / pathology*
  • Neoplastic Cells, Circulating

Substances

  • Antigens, CD19
  • DNA, Neoplasm
  • Fusion Proteins, bcr-abl