Abstract
Growth factor receptors are important determinants of both normal and abnormal cell growth. We have now used degenerate primers designed from conserved tyrosine kinase domains to identify and clone a novel receptor-like molecule (designated Nbtk-1) from a NB41 mouse neuroblastoma cell line. Nbtk-1 is related to the met proto-oncogene family of tyrosine kinase receptors. Transcripts of approximately 2.1 and 2.6 kb have been found in mouse cell lines and one transcript of approximately 3 kb in human cell lines and in a wide range of primary human tumors, such as neuroblastomas, primitive neuroectodermal tumors (PNETs), Wilms' tumors, and melanomas and in the corresponding normal human tissues. These observations suggest that Nbtk-1 may have important roles in normal and tumor cell growth.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Cloning, Molecular
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Humans
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Mice
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Molecular Sequence Data
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Neuroblastoma / enzymology*
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Neuroblastoma / genetics
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Poly A / genetics
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Poly A / isolation & purification
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Polymerase Chain Reaction
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Protein-Tyrosine Kinases / genetics*
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins c-met
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Proto-Oncogenes*
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger
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Receptors, Cell Surface / genetics*
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Sequence Homology, Amino Acid
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Transcription, Genetic
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Tumor Cells, Cultured
Substances
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MAS1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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RNA, Messenger
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Receptors, Cell Surface
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Poly A
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RNA
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Protein-Tyrosine Kinases
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Proto-Oncogene Proteins c-met
Associated data
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GENBANK/D13240
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GENBANK/D13241
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GENBANK/M96020
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GENBANK/M96021
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GENBANK/M96022
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GENBANK/M96023
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GENBANK/S52036
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GENBANK/S52789
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GENBANK/S52793
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GENBANK/S52794