Abstract
Islet amyloid polypeptide (IAPP) or Amylin is synthesized by the pancreatic beta-cells. IAPP is the major component of islet amyloid in the pancreas of patients with non-insulin-dependent diabetes mellitus. We report the composition and complete nucleotide sequence of the two human IAPP mRNAs of 1.6 and 2.1 kb. A new polyadenylation site was identified and shown to be used in generation of the 2.1 kb RNA. A previously identified polyadenylation signal is assigned to the 1.6 kb RNA. We exactly determined the major transcription start site, which is used in generation of these mRNAs. Lower abundance RNAs containing sequences located further upstream in the IAPP gene were also detected.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amyloid / genetics*
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Base Sequence
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Blotting, Southern
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Diabetes Mellitus, Type 2 / physiopathology
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Exons
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Gene Amplification
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Humans
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Insulinoma / genetics
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Introns
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Islet Amyloid Polypeptide
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Islets of Langerhans / physiology
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Pancreatic Neoplasms / genetics
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Poly A / genetics*
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Poly A / isolation & purification
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Polymerase Chain Reaction
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RNA / genetics*
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RNA / isolation & purification
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RNA, Messenger
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Transcription, Genetic*
Substances
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Amyloid
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Islet Amyloid Polypeptide
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Oligodeoxyribonucleotides
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RNA, Messenger
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Poly A
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RNA
Associated data
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GENBANK/D12944
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GENBANK/D12945
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GENBANK/D12946
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GENBANK/D12947
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GENBANK/D12948
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GENBANK/D12949
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GENBANK/D12950
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GENBANK/D12951
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GENBANK/X67120
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GENBANK/X68830