Abstract
To develop a high-titer surrogate virus for human T-cell leukemia virus type 1 (HTLV-1), we generated recombinant vesicular stomatitis viruses (VSVs) in which the gene encoding the single transmembrane glycoprotein (G) was deleted. Genes encoding HTLV-1 envelope glycoproteins (HTEnv) or HTEnvG hybrid proteins were then inserted into either of two different sites in the VSV genome. The viruses also encoded a green fluorescent protein. With this surrogate virus, we found that a soluble protein, osteoprotegerin (OPG), or an OPG/Fc chimeric protein inhibited the infection of various cell lines. Our experiments indicate that this inhibition resulted from binding of heparan sulfate by OPG.
Publication types
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Evaluation Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Cricetinae
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Gene Deletion
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Glycoproteins / metabolism
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Glycoproteins / pharmacology
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Heparitin Sulfate / metabolism
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Human T-lymphotropic virus 1 / genetics
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Human T-lymphotropic virus 1 / metabolism
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Human T-lymphotropic virus 1 / pathogenicity*
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Humans
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Membrane Glycoproteins / genetics
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Mice
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Osteoprotegerin
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Receptors, Cytoplasmic and Nuclear / metabolism
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Receptors, Tumor Necrosis Factor
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Recombination, Genetic
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Vesicular stomatitis Indiana virus / genetics*
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / metabolism*
Substances
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G protein, vesicular stomatitis virus
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Glycoproteins
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Membrane Glycoproteins
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Osteoprotegerin
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Receptors, Cytoplasmic and Nuclear
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Receptors, Tumor Necrosis Factor
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TNFRSF11B protein, human
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Tnfrsf11b protein, mouse
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Viral Envelope Proteins
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Heparitin Sulfate