Abstract
Angiogenesis plays an important role in tumor growth and metastasis and is regulated by a balance between angiogenic stimulators and inhibitors. We investigated the gene expression profile of vascular endothelial growth factor (VEGF) and pigment epithelium-derived factor (PEDF), a potent endogenous anti-angiogenic factor, in human oral squamous cell carcinoma (SCC) cell lines. The treatment of SCC cells with hypoxia increased the expression of PEDF as well as VEGF. Moreover, the treatment of SCC cells with VEGF enhanced the expression of PEDF mRNA and secretion of PEDF. In LMF-4, a SCC clone producing abundant VEGF and PEDF, the addition of neutralizing VEGF antibody substantially blocked PEDF expression. These data suggest that human oral squamous cell carcinoma cells produce VEGF, which in turn regulates PEDF production, and this balance may be contributing to neovascularization in tumors.
MeSH terms
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Angiogenesis Inhibitors
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Carcinoma, Squamous Cell / metabolism*
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Cell Hypoxia
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Endothelial Growth Factors / metabolism*
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Endothelial Growth Factors / pharmacology
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Eye Proteins*
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Gene Expression Regulation, Neoplastic
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Humans
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Intercellular Signaling Peptides and Proteins / metabolism*
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Intercellular Signaling Peptides and Proteins / pharmacology
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Lymphokines / metabolism*
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Lymphokines / pharmacology
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Mouth Neoplasms / metabolism*
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Nerve Growth Factors*
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Proteins / metabolism*
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Proteins / pharmacology*
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Serpins / metabolism*
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Tumor Cells, Cultured
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
Substances
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Angiogenesis Inhibitors
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Endothelial Growth Factors
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Eye Proteins
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Intercellular Signaling Peptides and Proteins
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Lymphokines
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Nerve Growth Factors
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Proteins
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Serpins
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Vascular Endothelial Growth Factor A
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Vascular Endothelial Growth Factors
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pigment epithelium-derived factor