Abstract
Rat T9.F glioma cells were transduced with the interleukin (IL)-2 gene. Clone T9.F/IL2/#12 secreted a high level of IL-2 (15 ng/10(6) cells/48 h). Enhanced tumor progression and reduced survival was observed when T9.F/IL2/#12 cells were implanted intracranially. Subcutaneous injection of T9.F/IL2/#12 cells induced a palpable nodule, which regressed in approximately 15 days, resulting in tumor-specific protection. Lymphocytes from T9.F/IL2/#12 primed rats specifically respond to T9.F antigens but lacked cytotoxicity towards T9.F cells. Intracranial T9.F/IL2/#12 tumors were markedly infiltrated by CD4(+) and CD8(+) T cells, natural killer (NK)-T cells and myeloid progenitor cells, whereas subcutaneous T9.F/IL2/#12 tumors contained an elevated level of NK cells.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Antigens, Neoplasm / administration & dosage
-
Antigens, Neoplasm / genetics
-
Brain Neoplasms / genetics
-
Brain Neoplasms / metabolism*
-
Brain Neoplasms / mortality
-
Brain Neoplasms / pathology
-
Cell Division / genetics
-
Cell Division / immunology
-
Cell Line
-
Female
-
Gene Transfer Techniques
-
Genetic Vectors
-
Glioma / genetics
-
Glioma / metabolism*
-
Glioma / mortality
-
Glioma / pathology
-
Graft Rejection / genetics
-
Graft Rejection / immunology*
-
Graft Rejection / pathology
-
Humans
-
Injections, Subcutaneous
-
Interleukin-2 / administration & dosage
-
Interleukin-2 / genetics
-
Interleukin-2 / metabolism*
-
Lymphocyte Subsets / immunology
-
Lymphocyte Subsets / pathology
-
Lymphocyte Subsets / transplantation
-
Lymphocytes, Tumor-Infiltrating / immunology
-
Lymphocytes, Tumor-Infiltrating / pathology
-
Myeloid Progenitor Cells / immunology
-
Myeloid Progenitor Cells / pathology
-
Neoplasm Transplantation
-
Rats
-
Rats, Inbred F344
-
Skin Neoplasms / genetics
-
Skin Neoplasms / immunology
-
Skin Neoplasms / prevention & control*
-
Tumor Cells, Cultured
Substances
-
Antigens, Neoplasm
-
Interleukin-2