Transcriptional regulation of human beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

Liming Xu; Yoshikazu Kurusu; Kaori Takizawa; Junzo Tanaka; Kojiro Matsumoto; Akiyoshi Taniguchi

doi:10.1016/s0006-291x(03)01314-7

Transcriptional regulation of human beta-galactoside alpha2,6-sialyltransferase (hST6Gal I) gene in colon adenocarcinoma cell line

Biochem Biophys Res Commun. 2003 Aug 8;307(4):1070-4. doi: 10.1016/s0006-291x(03)01314-7.

Authors

Liming Xu¹, Yoshikazu Kurusu, Kaori Takizawa, Junzo Tanaka, Kojiro Matsumoto, Akiyoshi Taniguchi

Affiliation

¹ Bionic Materials Technology Group, Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan.

PMID: 12878221
DOI: 10.1016/s0006-291x(03)01314-7

Abstract

Previous studies have shown that hST6Gal I mRNA is overexpressed in colorectal cancer tissues compared with non-malignant or benign tissue. Moreover, Form 1 (hepatic form) mRNA isoform had a marked tendency to accumulate in colon cancer [Int. J. Cancer 88 (2000) 58-65]. These findings suggest that the transcriptional regulation of Form 1 is altered during malignant transformation. We report here transcriptional regulation of the hST6Gal I gene in colon adenocarcinoma cell lines. We characterized P1 promoter region, which regulates Form 1 mRNA expression, using luciferase assays. The result indicates that the nt-156 to -1 region is important for transcriptional activity of hST6Gal I gene in colon adenocarcinoma cell lines. The nt-156 to -1 region contains HNF1 recognition element. Mutation of the HNF1 site reduced luciferase activity by about 80% compared with the wild-type construct, suggesting that HNF1 site is involved in the transcription of Form 1 mRNA in colon cancer cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenocarcinoma / genetics*
Adenocarcinoma / metabolism
Binding Sites
Colonic Neoplasms / genetics*
Colonic Neoplasms / metabolism
DNA Mutational Analysis
DNA-Binding Proteins*
Enzyme Induction
Gene Expression Regulation, Neoplastic*
Hepatocyte Nuclear Factor 1
Hepatocyte Nuclear Factor 1-alpha
Hepatocyte Nuclear Factor 1-beta
Humans
Isoenzymes / biosynthesis
Isoenzymes / genetics
Nuclear Proteins*
Promoter Regions, Genetic
RNA, Messenger / biosynthesis
Sialyltransferases / biosynthesis
Sialyltransferases / genetics*
Transcription Factors / metabolism
Transcriptional Activation
Tumor Cells, Cultured
beta-D-Galactoside alpha 2-6-Sialyltransferase

Substances

DNA-Binding Proteins
HNF1A protein, human
HNF1B protein, human
Hepatocyte Nuclear Factor 1-alpha
Isoenzymes
Nuclear Proteins
RNA, Messenger
Transcription Factors
Hepatocyte Nuclear Factor 1
Hepatocyte Nuclear Factor 1-beta
Sialyltransferases
beta-D-Galactoside alpha 2-6-Sialyltransferase