CD69-null mice protected from arthritis induced with anti-type II collagen antibodies

Kaoru Murata; Masamichi Inami; Akihiro Hasegawa; Shuichi Kubo; Motoko Kimura; Masakatsu Yamashita; Hiroyuki Hosokawa; Tomokazu Nagao; Kazuo Suzuki; Kahoko Hashimoto; Hiroshi Shinkai; Haruhiko Koseki; Masaru Taniguchi; Steven F Ziegler; Toshinori Nakayama

doi:10.1093/intimm/dxg102

CD69-null mice protected from arthritis induced with anti-type II collagen antibodies

Int Immunol. 2003 Aug;15(8):987-92. doi: 10.1093/intimm/dxg102.

Authors

Kaoru Murata¹, Masamichi Inami, Akihiro Hasegawa, Shuichi Kubo, Motoko Kimura, Masakatsu Yamashita, Hiroyuki Hosokawa, Tomokazu Nagao, Kazuo Suzuki, Kahoko Hashimoto, Hiroshi Shinkai, Haruhiko Koseki, Masaru Taniguchi, Steven F Ziegler, Toshinori Nakayama

Affiliation

¹ Department of Molecular Immunology and Medical Immunology, Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan.

PMID: 12882836
DOI: 10.1093/intimm/dxg102

Abstract

CD69, known as an early activation marker antigen on T and B cells, is also expressed on platelets and activated neutrophils, suggesting certain roles in inflammatory diseases. In order to address the role of CD69 in the pathogenesis of arthritis, we established CD69-null mice. CD69-null mice displayed a markedly attenuated arthritic inflammatory response when injected with anti-type II collagen antibodies. Cell transfer experiments with neutrophils, but not T cells or spleen cells, from wild-type mice into CD69-null mice restored the induction of arthritis. These results indicate a critical role for CD69 in neutrophil function in arthritis induction during the effector phase. Thus, CD69 would be a possible therapeutic target for arthritis in human patients.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Adoptive Transfer
Animals
Ankle Joint / metabolism
Ankle Joint / pathology
Antibodies, Monoclonal / pharmacology*
Antigens, CD / analysis
Antigens, CD / genetics
Antigens, CD / physiology*
Antigens, Differentiation, T-Lymphocyte / analysis
Antigens, Differentiation, T-Lymphocyte / genetics
Antigens, Differentiation, T-Lymphocyte / physiology*
Arthritis, Experimental / chemically induced*
Arthritis, Experimental / genetics
Arthritis, Experimental / pathology
Chemokines / genetics
Collagen Type II / immunology*
Cytokines / genetics
Female
Gene Expression Profiling
Hindlimb / metabolism
Hindlimb / pathology
Hindlimb / physiopathology
In Situ Hybridization / methods
Lectins, C-Type
Lipopolysaccharides / pharmacology
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Knockout
Neutrophils / drug effects
Neutrophils / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Thioglycolates / pharmacology

Substances

Antibodies, Monoclonal
Antigens, CD
Antigens, Differentiation, T-Lymphocyte
CD69 antigen
Chemokines
Collagen Type II
Cytokines
Lectins, C-Type
Lipopolysaccharides
Thioglycolates