Abstract
Inhibitory interneurons play important roles in neuronal circuits, but the synaptic mechanisms that regulate excitatory input onto interneurons remain to be fully understood. We show that ATP-gated presynaptic P2X2 channels facilitate excitatory transmission onto stratum radiatum interneurons but not onto CA1 pyramidal neurons. ATP released endogenously during carbachol-induced oscillations facilitates excitatory synapses onto interneurons. Overall, these data provide evidence for the molecular identity, synaptic function, and interneuron synapse specificity of a presynaptic neurotransmitter-gated cation channel. The findings highlight a novel form of presynaptic facilitation for hippocampal interneurons and suggest a role for extracellular ATP in neuronal networks.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphate / pharmacology*
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Animals
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Calcium Channels / metabolism
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Cells, Cultured
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Electric Conductivity
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Excitatory Postsynaptic Potentials
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Interneurons / physiology*
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Mice
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Mice, Knockout
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Nerve Net
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Patch-Clamp Techniques
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Periodicity
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Pyramidal Cells / chemistry
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Pyramidal Cells / cytology
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Pyramidal Cells / physiology*
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Receptors, Purinergic P2 / analysis
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Receptors, Purinergic P2 / genetics
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Receptors, Purinergic P2 / metabolism
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Receptors, Purinergic P2 / physiology*
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Receptors, Purinergic P2X2
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Signal Transduction
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Synapses / metabolism
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Synapses / physiology*
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Synaptic Transmission* / drug effects
Substances
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Calcium Channels
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P2rx2 protein, mouse
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Receptors, Purinergic P2
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Receptors, Purinergic P2X2
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Adenosine Triphosphate