Abstract
The human SYT-SSX gene has two splicing isoforms (type N and I), the latter of which contains an additional insertion of 93 bases. In the present study, we found increased transcriptional activity of the SYT-SSX type I protein in luciferase assay. When the SYT-SSX cDNAs were transfected to NIH3T3 cells, the type I transformant grew faster than the type N transformant. Furthermore, we evaluated the isoform ratio of the SYT or SYT-SSX transcripts in various tissues. Our results suggest that the SYT-SSX type I protein plays a critical role in the tumorigenesis of synovial sarcomas through increased transcriptional activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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3T3 Cells
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Alternative Splicing / genetics*
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Animals
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Cell Division / genetics
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Genes, Reporter
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Humans
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Luciferases / genetics
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Mice
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Mutagenesis, Insertional
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Neoplasm Proteins / genetics*
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Neoplasm Proteins / metabolism
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Protein Isoforms / genetics
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Proteins / genetics*
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Proteins / metabolism
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Proto-Oncogene Proteins
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins / genetics*
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Repressor Proteins / metabolism
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Sarcoma, Synovial / genetics
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Transcription, Genetic*
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Transcriptional Activation / genetics
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Transfection
Substances
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Neoplasm Proteins
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Protein Isoforms
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Proteins
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Proto-Oncogene Proteins
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Recombinant Fusion Proteins
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Repressor Proteins
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SS18 protein, human
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Ss18 protein, mouse
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synovial sarcoma X breakpoint proteins
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Luciferases