Abstract
Interleukin 9 (IL-9) is a T-cell-derived lymphokine that induces the proliferation of various lymphoid and hemopoietic cells. A cDNA clone encoding the murine IL-9 receptor was isolated by expression cloning in COS cells and screening with 125I-labeled IL-9. Transient expression of this cDNA produced high-affinity binding sites for IL-9. The predicted 52-kDa protein contains a putative signal peptide and a typical transmembrane domain. A cDNA for the human homologue was isolated by cross-hybridization. Transfection of this cDNA in a murine T-cell clone conferred responsiveness to human IL-9. Sequence analysis revealed that the IL-9 receptor belongs to the recently described hematopoietin receptor super-family and is expressed in membrane-bound and soluble forms.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cell Line
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Cloning, Molecular
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DNA / genetics*
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DNA / isolation & purification
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Gene Library
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Humans
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Interleukin-9 / metabolism*
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Kinetics
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Mice
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Molecular Sequence Data
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Poly A / genetics
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Poly A / isolation & purification
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RNA / genetics
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RNA / isolation & purification
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RNA, Messenger
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Receptors, Immunologic / genetics*
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Receptors, Immunologic / metabolism
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Receptors, Interleukin*
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Receptors, Interleukin-9
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Recombinant Proteins / metabolism
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Sequence Homology, Nucleic Acid
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T-Lymphocytes / immunology
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Transfection
Substances
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IL9R protein, human
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Interleukin-9
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RNA, Messenger
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Receptors, Immunologic
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Receptors, Interleukin
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Receptors, Interleukin-9
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Recombinant Proteins
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Poly A
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RNA
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DNA
Associated data
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GENBANK/M84746
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GENBANK/M84747