Cyclin B is associated with centrosomes in Drosophila mitotic cells

Biol Cell. 1992;75(2):121-6. doi: 10.1016/0248-4900(92)90131-j.

Abstract

We have studied by way of confocal laser scanning microscopy the subcellular localization of cyclin B in Drosophila-cultured cells and report here evidence that a part of the cyclin B cell pool is closely associated with the centrosome. This cyclin B centrosomal signal is strong in prophase and metaphase but disappears during anaphase. Moreover, the signal is absent in the acentriolar Drosophila cell line 1182-4. These results put forward additional arguments suggesting that the centrosome plays an important role in the control of the cell cycle.

MeSH terms

  • Animals
  • Blotting, Western
  • Cell Line
  • Centrioles / metabolism*
  • Cyclins / metabolism*
  • Drosophila melanogaster
  • Fluorescent Antibody Technique
  • Mitosis

Substances

  • Cyclins