Human ribosomal protein S3a: cloning of the cDNA and primary structure of the protein

Gene. 1992 Oct 1;119(2):313-6. doi: 10.1016/0378-1119(92)90289-2.

Abstract

The amino acid (aa) sequence of human ribosomal protein S3a (hRPS3a) was deduced partially from the nucleotide sequence of the corresponding cDNA and confirmed by direct aa sequencing from the N terminus of the purified hRPS3a protein. The cDNA clone was isolated from a cDNA expression library in the pEX vector using antibodies. The hRPS3a protein has 263 aa and its calculated M(r) is 29 813.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • Cloning, Molecular
  • DNA
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • RNA, Messenger / genetics
  • Ribosomal Proteins / genetics*

Substances

  • RNA, Messenger
  • RPS3A protein, human
  • Ribosomal Proteins
  • DNA

Associated data

  • GENBANK/M77234