Abstract
The product of adenovirus type 5 (Ad5) gene IX, protein IX (pIX), is a multifunctional protein that stabilizes the viral capsid and has transcriptional activity. We show that pIX also contributes to the Ad5-induced reorganization of the host-cell nuclear ultrastructure: pIX induces the formation of specific and dynamic nuclear inclusions, and the host promyelocytic leukaemia (PML) protein, which is the main structural organizer of PML bodies, is stably relocated and confined within the pIX-induced inclusions late in infection. Our results suggest that Ad5 has evolved a unique strategy that leads to the sustained neutralization of PML bodies throughout infection, thereby ensuring optimal viral proliferation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics
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Adenoviridae / physiology*
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Adenoviridae Infections
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Capsid Proteins / chemistry
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Capsid Proteins / genetics
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Capsid Proteins / metabolism*
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Cell Line
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Humans
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Intranuclear Inclusion Bodies / metabolism
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Intranuclear Inclusion Bodies / ultrastructure
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Neoplasm Proteins / metabolism*
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Nuclear Proteins / metabolism*
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Promyelocytic Leukemia Protein
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Protein Structure, Tertiary
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Transcription Factors / metabolism*
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Tumor Suppressor Proteins
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Virus Replication*
Substances
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Capsid Proteins
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Neoplasm Proteins
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Nuclear Proteins
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Promyelocytic Leukemia Protein
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Recombinant Proteins
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Transcription Factors
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Tumor Suppressor Proteins
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hexon capsid protein, Adenovirus
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PML protein, human