Xenotransplantation of embryonic precursors of human myogenesis for the correction of dystrophinopathy in mice with hereditary muscular dystrophy

V N Yarygin; G T Sukhikh; V F Sitnikov; M A Stenina; A B Kuznetsov; R A Poltavtseva; L I Krivov; D A Voevodin; V I Savchuk; A V Revishchin; L I Korochkin; M A Aleksandrova

doi:10.1023/a:1026009517850

Xenotransplantation of embryonic precursors of human myogenesis for the correction of dystrophinopathy in mice with hereditary muscular dystrophy

Bull Exp Biol Med. 2003 Jul;136(1):88-92. doi: 10.1023/a:1026009517850.

Authors

V N Yarygin¹, G T Sukhikh, V F Sitnikov, M A Stenina, A B Kuznetsov, R A Poltavtseva, L I Krivov, D A Voevodin, V I Savchuk, A V Revishchin, L I Korochkin, M A Aleksandrova

Affiliation

¹ Russian State Medical University, Moscow.

PMID: 14534620
DOI: 10.1023/a:1026009517850

Abstract

Human embryonic myogenic precursors were transplanted into muscles of mdx mice with hereditary dystrophin-deficient muscular dystrophy. Transplantation induced the synthesis of human dystrophin. The number of dystrophin-positive fibers progressively decreased, however, some of them were preserved even 5 months after transplantation. Our results indicate that xenogeneic transplantation of embryonic myogenic precursors compensates the genetic defect in dystrophin-deficient mice.

MeSH terms

Animals
Cell Transplantation
DNA / metabolism
Disease Models, Animal
Dystrophin / genetics
Dystrophin / metabolism
Embryo, Mammalian / cytology*
Exons
Genotype
Heterozygote
Homozygote
Humans
Immunohistochemistry
Mice
Mice, Inbred mdx
Muscles / cytology*
Muscular Dystrophies / genetics
Muscular Dystrophies / pathology*
Muscular Dystrophy, Animal / genetics
Muscular Dystrophy, Animal / pathology*
Mutation
Polymerase Chain Reaction
Time Factors
Transplantation, Heterologous

Substances

Dystrophin
DNA