[Detecting MTHFR gene mutation with a new approach: molecular beacons]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2003 Oct;20(5):436-7.
[Article in Chinese]

Abstract

Objective: To detect the methylenetetrahydrofolate reductase(MTHFR) gene C677T mutation with molecular beacon technique and assess the revant applicability.

Methods: A total of 228 samples were analyzed using molecular beacons which are oligonucleotide probes to become fluorescent upon hybridization. Wild-type molecular beacon and mutant beacon were designed to detect the genotypes of MTHFR gene.

Results: Analysis of the 228 samples indicated that there were three genotypes including 41 homozygous mutants, 113 heterozygous individuals and 74 wild-type individuals. Every sample was identified clearly.

Conclusion: The present method, a closed-tube PCR/hybridization assay, is a simple, high-throughput and fast procedure that is fully automated for detecting gene mutation.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Mutational Analysis / methods
  • Fluorescent Dyes / chemistry
  • Genotype
  • Humans
  • Methylenetetrahydrofolate Reductase (NADPH2) / genetics*
  • Oligonucleotide Probes / chemistry
  • Oligonucleotide Probes / genetics
  • Point Mutation*
  • Sensitivity and Specificity

Substances

  • Fluorescent Dyes
  • Oligonucleotide Probes
  • Methylenetetrahydrofolate Reductase (NADPH2)