Abstract
The expression of Gas6, the protein product of the growth arrest-specific gene 6 (gas6), a member of the vitamin K-dependent protein family, and the receptor tyrosine kinases Axl and Sky and their mRNAs in uterine leiomyoma and normal uterine myometrium tissues were investigated by competitive RT-PCR-Southern blot analysis using recombinant RNA and immuno histochemical analysis respectively. There was no significant difference between the histoscores and levels of Sky mRNA in uterine leiomyoma and normal uterine myometrium, although the levels of Gas6 and Axl mRNAs in uterine leiomyoma were significantly higher than in normal uterine myometrium in each case. It is suggested that Gas6 and Axl signal transduction is aberrantly stimulated in uterine leiomyoma, possibly related to its growth.
MeSH terms
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Adult
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Axl Receptor Tyrosine Kinase
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Female
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Humans
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Intercellular Signaling Peptides and Proteins / genetics
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Intercellular Signaling Peptides and Proteins / metabolism*
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Leiomyoma / diagnosis*
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Leiomyoma / enzymology
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Leiomyoma / metabolism
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Myometrium / cytology
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Myometrium / enzymology
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Myometrium / metabolism
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Oncogene Proteins / genetics
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Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins
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RNA, Messenger / metabolism
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Receptor Protein-Tyrosine Kinases / genetics
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Receptor Protein-Tyrosine Kinases / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Uterine Neoplasms / diagnosis*
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Uterine Neoplasms / enzymology
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Uterine Neoplasms / metabolism
Substances
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Intercellular Signaling Peptides and Proteins
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Oncogene Proteins
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Proto-Oncogene Proteins
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RNA, Messenger
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growth arrest-specific protein 6
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Receptor Protein-Tyrosine Kinases
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TYRO3 protein, human
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Axl Receptor Tyrosine Kinase
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AXL protein, human