Abstract
Five sequential Cryptococcus neoformans isolates recovered from an AIDS patient with recurrent meningitis were analyzed. Four isolates were fluconazole susceptible, while the fifth isolate developed fluconazole resistance. Analysis of the 14-alpha lanosterol demethylase gene (ERG11) showed a point mutation in the resistant strain responsible for the amino acid substitution G484S.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acquired Immunodeficiency Syndrome / complications
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Adult
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Amino Acid Sequence
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Amino Acid Substitution
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Antifungal Agents / pharmacology*
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Cloning, Molecular
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Cryptococcus neoformans / drug effects*
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Cytochrome P-450 Enzyme System / genetics*
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Drug Resistance, Fungal / genetics*
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Fluconazole / pharmacology*
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Genotype
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Humans
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Male
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Meningitis, Cryptococcal / microbiology*
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Microbial Sensitivity Tests
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Molecular Sequence Data
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Oxidoreductases / genetics*
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Plasmids
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Point Mutation / genetics
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Recurrence
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Sterol 14-Demethylase
Substances
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Antifungal Agents
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CYP51A1 protein, human
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Fluconazole
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Cytochrome P-450 Enzyme System
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Oxidoreductases
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Sterol 14-Demethylase