Abstract
Osteogenic protein-2, OP-2, a new member of the transforming growth factor-beta (TGF-beta) superfamily, closely related to the osteogenic/bone morphogenetic proteins, was discovered in mouse embryo and human hippocampus cDNA libraries. The TGF-beta domain of OP-2 shows 74% identity to OP-1, 75% to Vgr-1, and 76% to BMP-5, hence OP-2 may also have bone inductive activity. The genomic locus of OP-2 has seven exons, like OP-1, and spans more than 27 kilobases (kb). In the C-terminal TGF-beta domain, OP-2 has a unique additional cysteine. Mouse embryos express relatively high levels of OP-2 mRNA at 8 days, two species of 3 and 5 kb. A careful study of mRNA expression of the osteogenic proteins in specific organs revealed discrete mRNA species for BMP-3, BMP-4, BMP-5, and BMP-6/Vgr-1 in lung or liver of young and adult mice. OP-1 is expressed in kidney; however, OP-2 and BMP-2 mRNAs were not detected in any organs studied, suggesting an early developmental role.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Animals, Newborn
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Base Sequence
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Proteins*
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Cloning, Molecular
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DNA / genetics
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DNA / isolation & purification
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Embryo, Mammalian
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Embryonic and Fetal Development*
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Exons
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Gene Expression
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Gene Library
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Hippocampus / physiology*
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Humans
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Mice
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Molecular Sequence Data
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Multigene Family*
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Oligodeoxyribonucleotides
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Organ Specificity
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Protein Precursors / genetics
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Proteins / genetics*
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Transforming Growth Factor beta / genetics*
Substances
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BMP2 protein, human
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BMP8B protein, human
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Bmp2 protein, mouse
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Bmp8b protein, mouse
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Bone Morphogenetic Protein 2
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Bone Morphogenetic Proteins
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Oligodeoxyribonucleotides
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Protein Precursors
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Proteins
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RNA, Messenger
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Transforming Growth Factor beta
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DNA
Associated data
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GENBANK/D10519
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GENBANK/D10520
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GENBANK/D12749
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GENBANK/D12750
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GENBANK/D12751
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GENBANK/D12752
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GENBANK/D12753
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GENBANK/M83144
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GENBANK/M97016
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GENBANK/M97017