Osteogenic protein-2. A new member of the transforming growth factor-beta superfamily expressed early in embryogenesis

J Biol Chem. 1992 Dec 15;267(35):25220-7.

Abstract

Osteogenic protein-2, OP-2, a new member of the transforming growth factor-beta (TGF-beta) superfamily, closely related to the osteogenic/bone morphogenetic proteins, was discovered in mouse embryo and human hippocampus cDNA libraries. The TGF-beta domain of OP-2 shows 74% identity to OP-1, 75% to Vgr-1, and 76% to BMP-5, hence OP-2 may also have bone inductive activity. The genomic locus of OP-2 has seven exons, like OP-1, and spans more than 27 kilobases (kb). In the C-terminal TGF-beta domain, OP-2 has a unique additional cysteine. Mouse embryos express relatively high levels of OP-2 mRNA at 8 days, two species of 3 and 5 kb. A careful study of mRNA expression of the osteogenic proteins in specific organs revealed discrete mRNA species for BMP-3, BMP-4, BMP-5, and BMP-6/Vgr-1 in lung or liver of young and adult mice. OP-1 is expressed in kidney; however, OP-2 and BMP-2 mRNAs were not detected in any organs studied, suggesting an early developmental role.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Newborn
  • Base Sequence
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins*
  • Cloning, Molecular
  • DNA / genetics
  • DNA / isolation & purification
  • Embryo, Mammalian
  • Embryonic and Fetal Development*
  • Exons
  • Gene Expression
  • Gene Library
  • Hippocampus / physiology*
  • Humans
  • Mice
  • Molecular Sequence Data
  • Multigene Family*
  • Oligodeoxyribonucleotides
  • Organ Specificity
  • Protein Precursors / genetics
  • Proteins / genetics*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Sequence Homology, Amino Acid
  • Transforming Growth Factor beta / genetics*

Substances

  • BMP2 protein, human
  • BMP8B protein, human
  • Bmp2 protein, mouse
  • Bmp8b protein, mouse
  • Bone Morphogenetic Protein 2
  • Bone Morphogenetic Proteins
  • Oligodeoxyribonucleotides
  • Protein Precursors
  • Proteins
  • RNA, Messenger
  • Transforming Growth Factor beta
  • DNA

Associated data

  • GENBANK/D10519
  • GENBANK/D10520
  • GENBANK/D12749
  • GENBANK/D12750
  • GENBANK/D12751
  • GENBANK/D12752
  • GENBANK/D12753
  • GENBANK/M83144
  • GENBANK/M97016
  • GENBANK/M97017