Abstract
Escherichia coli (thyA DeltafolA) mutants are viable and can grow in minimal medium when supplemented with thymidine alone. Here we present evidence from in vivo and in vitro studies that the ydgB gene determines an alternative dihydrofolate reductase that is related to the trypanosomatid pteridine reductases. We propose to rename this gene folM.
Publication types
-
Comparative Study
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Amino Acid Sequence
-
Biopterins / metabolism
-
Escherichia coli / enzymology*
-
Escherichia coli Proteins / chemistry
-
Escherichia coli Proteins / genetics*
-
Molecular Sequence Data
-
Mutation
-
Sequence Alignment
-
Tetrahydrofolate Dehydrogenase / chemistry
-
Tetrahydrofolate Dehydrogenase / genetics*
-
Tetrahydrofolate Dehydrogenase / metabolism
-
Tetrahydrofolates / metabolism
Substances
-
Escherichia coli Proteins
-
Tetrahydrofolates
-
Biopterins
-
5,6,7,8-tetrahydrofolic acid
-
FolM protein, E coli
-
Tetrahydrofolate Dehydrogenase