Abstract
Previous gene function analyses have indicated that HOXA9, DEK, CBL and CSF1R are aberrantly expressed in acute myeloid leukemia (AML). We analyzed the expression of these genes in a series of 41 adult patients with AML using quantitative real-time RT-PCR, and tested the association of the expression with the following hematologic and clinical parameters: age, FAB, immunophenotype and karyotype aberrations. A high proportion of the patients showed over- or underexpression of the analyzed genes. DEK was overexpressed in 98% of the cases, whereas CBL, CSF1R and HOXA9 were either overexpressed in 20%, 17% and 78% or underexpressed in 20%, 42% and 15% of the cases, respectively. Patients whose karyotype contained t(8;21)(q22;q22), showed lower relative expression of HOXA9 at a statistically significant level (p < 0.05). Bone marrow samples without expression of CD34 antigen were associated with either overexpression of DEK or HOXA9. Furthermore, an association was found between the AML-M2 subtype and lower expression of CBL, CSF1R or HOXA9, and between the AML-M5 subtype and CBL or CSF1R overexpression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acute Disease
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Adult
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Aged
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Antigens, CD / metabolism
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Chromosomal Proteins, Non-Histone*
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Chromosomes, Human, Pair 21 / genetics
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Chromosomes, Human, Pair 8 / genetics
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Female
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Gene Expression Regulation, Leukemic
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Homeodomain Proteins / analysis
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Homeodomain Proteins / genetics*
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Humans
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Immunophenotyping
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Karyotyping
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Leukemia, Myeloid / genetics*
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Male
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Middle Aged
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Neoplasm Proteins / analysis
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Neoplasm Proteins / genetics
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Oncogene Protein v-cbl
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Oncogene Proteins / analysis
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Oncogene Proteins / genetics*
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Poly-ADP-Ribose Binding Proteins
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Protein-Tyrosine Kinases / analysis
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Protein-Tyrosine Kinases / genetics
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RNA, Messenger / genetics
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RNA, Neoplasm / genetics
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RNA, Neoplasm / metabolism
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Receptor, Macrophage Colony-Stimulating Factor / analysis
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Receptor, Macrophage Colony-Stimulating Factor / genetics*
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Retroviridae Proteins, Oncogenic / analysis
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Retroviridae Proteins, Oncogenic / genetics*
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Reverse Transcriptase Polymerase Chain Reaction
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Translocation, Genetic
Substances
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Antigens, CD
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Chromosomal Proteins, Non-Histone
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DEK protein, human
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Homeodomain Proteins
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Neoplasm Proteins
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Oncogene Protein v-cbl
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Oncogene Proteins
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Poly-ADP-Ribose Binding Proteins
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RNA, Messenger
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RNA, Neoplasm
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Retroviridae Proteins, Oncogenic
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homeobox protein HOXA9
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Protein-Tyrosine Kinases
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Receptor, Macrophage Colony-Stimulating Factor