Evaluation of HER-2/neu status by real-time quantitative PCR in malignant cartilaginous tumors

Int J Oncol. 2004 Mar;24(3):575-80.

Abstract

The expression of HER-2/neu has been proposed to be a prognostic indicator in osteosarcoma. To clarify the actual frequency of HER-2/neu expression in primary malignant cartilaginous tumors, we examined 89 cases comprising 17 conventional chondrosarcomas, 33 mesenchymal chondrosarcomas, and 39 clear cell chondrosarcomas. We used real-time PCR (LightCycler) assay to quantify the HER-2/neu gene status. The crossing point of HER-2 in normal control bone was 27.77. The crossing points of HER-2 in conventional, mesenchymal, and clear cell chondrosarcomas were 28.48+/-1.79, 27.74+/-3.02, 28.57+/-1.54, respectively. In conclusion, the amplification and overexpression of the HER-2/neu oncogene is absent or at least very rare in malignant cartilaginous tumors. The level of expression of HER-2/neu was similar in all cartilaginous tumor types.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chondrosarcoma / metabolism*
  • Chondrosarcoma, Mesenchymal / metabolism*
  • DNA / metabolism
  • DNA Primers / pharmacology
  • Genes, erbB-2 / genetics
  • Humans
  • Prognosis
  • Receptor, ErbB-2 / biosynthesis*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma, Clear Cell / metabolism*

Substances

  • DNA Primers
  • DNA
  • Receptor, ErbB-2