Association of endothelial constitutive nitric oxide synthase gene polymorphism with acute coronary syndrome in Koreans

Heart. 2004 Mar;90(3):282-5. doi: 10.1136/hrt.2002.003616.

Abstract

Objective: To examine the effects of two polymorphisms of the endothelial constitutive nitric oxide synthase (ecNOS) gene, 4a/4b(A:B) located in intron 4 and Glu298Asp(G:T) located in exon 7, on the development of acute coronary syndromes (ACS).

Methods: 164 patients with ACS and 142 control participants were investigated for genotype and conventional risk factors. Genotype was determined by polymerase chain reaction and restriction fragment length polymorphism analysis.

Results: Genotype and allele frequencies of the A:B polymorphism in the ACS group (0.15:0.85 for AA+AB:BB, 0.09:0.91 for A:B) differed from those in the control group (0.26:0.74 for AA+AB:BB, 0.15:0.85 for A:B). However, genotype and allele frequencies of the G:T polymorphism in the ACS group (0.22:0.78 for TT+TG:GG, 0.11:0.89 for T:G) were similar to those in the control group (0.17:0.83 for TT+TG:GG, 0.09:0.91 for T:G). Multiple logistic regression analysis showed that the non-BB (AA+AB) and the non-BB+GG genotypes were significant protective factors against ACS (odds ratios 0.49 and 0.34, 95% confidence intervals 0.26 to 0.93 and 0.14 to 0.83, respectively). In addition, linear association analysis showed that the percentage of ACS patients was significantly lower in the genotype group non-BB+GG than in the genotype group BB+non-GG (39.6% v 62.7%, p = 0.01).

Conclusions: The non-BB genotype of the ecNOS 4a/4b gene polymorphism is a protective factor against the development of ACS. The GG genotype of the ecNOS Glu298Asp polymorphism exerts a benefit in addition to the non-BB genotype in the Korean population.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Angina, Unstable / genetics*
  • Case-Control Studies
  • Female
  • Genotype
  • Humans
  • Korea
  • Male
  • Middle Aged
  • Myocardial Infarction / genetics*
  • Nitric Oxide Synthase / genetics*
  • Nitric Oxide Synthase Type III
  • Odds Ratio
  • Polymorphism, Genetic / genetics*
  • Polymorphism, Restriction Fragment Length
  • Regression Analysis
  • Risk Factors

Substances

  • NOS3 protein, human
  • Nitric Oxide Synthase
  • Nitric Oxide Synthase Type III