Evaluation of groEL gene analysis for identification of Borrelia burgdorferi sensu lato

J Clin Microbiol. 2004 Mar;42(3):1270-3. doi: 10.1128/JCM.42.3.1270-1273.2004.

Abstract

The nucleotide sequences of the groEL genes, the flagellin genes, and the 16S rRNA genes from 22 reference strains of Borrelia were compared. groEL sequence analysis is useful not only in interspecies differentiation but also in intraspecies differentiation of Borrelia afzelii and Borrelia garinii isolates.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Borrelia / classification
  • Borrelia / genetics
  • Borrelia burgdorferi / classification
  • Borrelia burgdorferi / genetics*
  • Borrelia burgdorferi / isolation & purification
  • Chaperonin 60 / genetics*
  • DNA Primers
  • Flagellin / genetics
  • Humans
  • Ixodes / microbiology
  • Lyme Disease / diagnosis
  • Phylogeny
  • Polymerase Chain Reaction
  • Skin / microbiology
  • Species Specificity

Substances

  • Chaperonin 60
  • DNA Primers
  • Flagellin