Cloning and characterization of a novel splicing variant of the ZADH1 gene

Cytogenet Genome Res. 2003;103(1-2):79-83. doi: 10.1159/000076293.

Abstract

We report here the cloning and characterization of a novel splicing variant of the human zinc binding alcohol dehydrogenase, domain containing 1 (ZADH1) gene. ZADH1 is localized on chromosome 14q24.2. The cDNA of this splicing variant is 1613 base pairs in length, and encodes a 351-amino acid protein with a putative molecular weight of 38.5 kDa. We named the novel splicing variant ZADH1b. By MTC- panel PCR analysis, it was found that ZADH1b was widely expressed in human tissues. Computer analysis revealed ZADH1 had a potential ADH_zinc_N domain and it had considerable homology with some dehydrogenases. It was speculated that ZADH1 may have definite metabolic roles in vivo as a dehydrogenase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 15-Oxoprostaglandin 13-Reductase
  • Alcohol Dehydrogenase / chemistry
  • Alcohol Dehydrogenase / genetics*
  • Alcohol Dehydrogenase / metabolism
  • Amino Acid Sequence
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Humans
  • Molecular Sequence Data
  • RNA Splice Sites
  • RNA Splicing*
  • Sequence Alignment
  • Tissue Distribution

Substances

  • DNA, Complementary
  • RNA Splice Sites
  • Alcohol Dehydrogenase
  • 15-Oxoprostaglandin 13-Reductase
  • PTGR2 protein, human