Abstract
The NF-kappa B subunits p50 and p65 and the product of the rel proto-oncogene are members of a growing class of transcription factors with a unique DNA-binding and dimerization domain. Nuclear transfer of each of these factors is controlled by cytoplasmic inhibitors, and regulated by specific stimuli. The inhibitors I kappa B-alpha and -beta and pp40 recognize either p65 or the c-rel protein. We show here that the proto-oncogene bcl-3, believed to be involved in certain human B-cell leukaemias, encodes a protein that functions as an I kappa B-like molecule for native NF-kappa B but is specific for the p50 subunit. The ankyrin repeat domain of the bcl-3 product is shown to mediate complex formation with NF-kappa B dimers by contracting the conserved dimerization domain of NF-kappa B.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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B-Cell Lymphoma 3 Protein
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Cell Nucleus / metabolism
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Chromatography, Affinity
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Chromosome Deletion
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Cloning, Molecular
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DNA-Binding Proteins / metabolism*
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HeLa Cells
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Host Cell Factor C1
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Humans
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Macromolecular Substances
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NF-kappa B / antagonists & inhibitors*
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Octamer Transcription Factor-1
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Proto-Oncogene Mas
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Proto-Oncogene Proteins / genetics*
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogenes*
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Recombinant Proteins / metabolism
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Repetitive Sequences, Nucleic Acid
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription Factors / metabolism*
Substances
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B-Cell Lymphoma 3 Protein
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BCL3 protein, human
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DNA-Binding Proteins
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HCFC1 protein, human
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Host Cell Factor C1
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MAS1 protein, human
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Macromolecular Substances
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NF-kappa B
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Octamer Transcription Factor-1
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POU2F1 protein, human
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Proto-Oncogene Mas
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Proto-Oncogene Proteins
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Recombinant Proteins
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Transcription Factors
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Tetradecanoylphorbol Acetate