Abstract
ZW10, a dynamitin-interacting protein associated with kinetochores, is known to participate directly in turning off of the spindle checkpoint. In the present study, we show that ZW10 is located in the endoplasmic reticulum as well as in the cytosol during interphase, and forms a subcomplex with RINT-1 (Rad50-interacting protein) and p31 in a large complex comprising syntaxin 18, an endoplasmic reticulum-localized t-SNARE implicated in membrane trafficking. Like conventional syntaxin-binding proteins, ZW10, RINT-1 and p31 dissociated from syntaxin 18 upon Mg(2+)-ATP treatment in the presence of NSF and alpha-SNAP, whereas the subcomplex was not disassembled. Overexpression, microinjection and knockdown experiments revealed that ZW10 is involved in membrane trafficking between the endoplasmic reticulum and Golgi. The present results disclose an unexpected role for a spindle checkpoint protein, ZW10, during interphase.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Cell Cycle Proteins / metabolism
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Cell Line
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Dynactin Complex
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Dyneins / metabolism
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Endoplasmic Reticulum / genetics
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Endoplasmic Reticulum / metabolism*
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Gene Expression Regulation
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Golgi Apparatus / genetics
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Golgi Apparatus / metabolism*
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Humans
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Interphase
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Intracellular Membranes / metabolism*
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins / genetics
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Membrane Proteins / metabolism
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Microtubule-Associated Proteins / metabolism
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Nuclear Proteins
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Protein Binding
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Protein Transport
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Qa-SNARE Proteins
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Two-Hybrid System Techniques
Substances
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Carrier Proteins
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Cell Cycle Proteins
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DCTN2 protein, human
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Dynactin Complex
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Intracellular Signaling Peptides and Proteins
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Membrane Proteins
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Microtubule-Associated Proteins
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Nuclear Proteins
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Qa-SNARE Proteins
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RINT1 protein, human
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ZWINT protein, human
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Dyneins