Abstract
The BDProbeTec MTB assay for direct detection of Mycobacterium tuberculosis was evaluated in comparison with the AMTD-II assay on 94 samples from different patients with clinical suspicion of tuberculosis. Using a combination of culture on Lowenstein-Jensen medium (with or without preculture in BACTEC 9000) and clinical diagnosis as the standard, BDProbeTec MTB showed high sensitivity and specificity (96.1% and 100%, respectively), similar to AMTD-II (96.1% and 97.1%, respectively), with significantly higher sensitivity than the Ziehl-Neelsen stain for acid-fast bacilli (73%, p < 0.05).
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Culture Media
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DNA Transposable Elements / genetics
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DNA, Ribosomal / genetics
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Humans
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Mycobacterium tuberculosis / genetics
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Mycobacterium tuberculosis / growth & development
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Mycobacterium tuberculosis / isolation & purification*
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Nucleic Acid Amplification Techniques / methods*
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RNA, Ribosomal, 16S / genetics
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Reagent Kits, Diagnostic
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Sensitivity and Specificity
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Tuberculosis / diagnosis*
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Tuberculosis / microbiology
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Tuberculosis, Pulmonary / diagnosis*
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Tuberculosis, Pulmonary / microbiology
Substances
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Culture Media
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DNA Transposable Elements
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DNA, Ribosomal
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RNA, Ribosomal, 16S
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Reagent Kits, Diagnostic