Abstract
Eight patients with paraneoplastic cerebellar degeneration (PCD) and anti-Yo antibodies were investigated to determine whether there is any association between cytotoxic T lymphocyte (CTL) responses reactive with two previously defined Yo-derived, HLA-A2.1 restricted epitopes (cdr2-1 and cdr2-2) and the presence of tumour-infiltrating CD8+ CTLs. cdr2-1 and cdr2-2-specific CTL responses could not be detected in 5 HLA-A2.1(+) patients in an ex vivo interferon-gamma ELISPOT assay and only 2/9 tumour sections contained CD8(+) intratumoural lymphocytes suggesting a very limited role for CTL-mediated tumour immunity in this patient group, all of whom had evidence of widespread malignancy at the time of diagnosis and/or death.
MeSH terms
-
Antibodies, Neoplasm / blood*
-
Breast Neoplasms / immunology
-
Cerebellar Neoplasms / immunology
-
Cytotoxicity Tests, Immunologic
-
DNA-Binding Proteins / immunology*
-
Enzyme-Linked Immunosorbent Assay / methods
-
Epitopes, T-Lymphocyte / immunology
-
Female
-
HLA-A2 Antigen / immunology
-
Humans
-
Immunohistochemistry
-
Interferon-gamma / analysis*
-
Interferon-gamma / metabolism
-
Lymphocyte Count
-
Lymphocytes, Tumor-Infiltrating / chemistry
-
Lymphocytes, Tumor-Infiltrating / immunology
-
Lymphocytes, Tumor-Infiltrating / pathology
-
Neoplasm Proteins / immunology*
-
Nerve Tissue Proteins / immunology
-
Ovarian Neoplasms / immunology
-
Paraneoplastic Cerebellar Degeneration / diagnosis
-
Paraneoplastic Cerebellar Degeneration / immunology*
-
Paraneoplastic Cerebellar Degeneration / pathology
-
Protein Isoforms / immunology
-
Staining and Labeling
-
T-Lymphocytes, Cytotoxic / immunology*
-
T-Lymphocytes, Cytotoxic / pathology
Substances
-
Antibodies, Neoplasm
-
CDR2 protein, human
-
DNA-Binding Proteins
-
Epitopes, T-Lymphocyte
-
HLA-A2 Antigen
-
Neoplasm Proteins
-
Nerve Tissue Proteins
-
Protein Isoforms
-
Interferon-gamma