Short-term down-regulation of the brain-specific, PtdIns(3,4,5)P3/Ins(1,3,4,5)P4-binding, adapter protein, p42IP4/centaurin-alpha 1 in rat brain after acoustic and electric stimulation

Neurochem Int. 2004 Jul;45(1):89-93. doi: 10.1016/j.neuint.2003.12.003.

Abstract

The protein p42(IP4), expressed mainly in brain, specifically recognizes two second messenger molecules, Ins(1,3,4,5)P(4) (IP(4)), a water soluble metabolite of IP(3) and the lipid PtdIns(3,4,5)P(3) (PIP(3)), the product of the growth factor-activated enzyme PI-3-kinase. Here, we studied whether there is short-term regulation of the expression level of p42(IP4) in limbic brain areas following acoustic and electric stimulation. The stimuli down-regulated the mRNA and protein levels within 2h in amygdala, hypothalamus and cingulate/retrospenial cortex. p42(IP4) mRNA decreased by about 50% for about 24h, but recovered to control values after 72 h. The present results are the first indication of a specific role of p42(IP4) in the short-term regulation of a behavioral response. They indicate that p42(IP4), an adapter protein in PIP(3)-dependent cellular signaling, may play an important role in the signal transduction pathways regulating plasticity in neuronal cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acoustic Stimulation / methods*
  • Adaptor Proteins, Signal Transducing
  • Adaptor Proteins, Vesicular Transport / antagonists & inhibitors*
  • Adaptor Proteins, Vesicular Transport / metabolism
  • Animals
  • Brain / metabolism*
  • Calcium Channels / metabolism
  • Carrier Proteins / antagonists & inhibitors*
  • Carrier Proteins / metabolism
  • Down-Regulation / physiology*
  • Electric Stimulation / methods
  • GTPase-Activating Proteins
  • Inositol 1,4,5-Trisphosphate Receptors
  • Inositol Phosphates / metabolism*
  • Male
  • Nerve Tissue Proteins / antagonists & inhibitors*
  • Nerve Tissue Proteins / metabolism
  • Neuronal Plasticity / physiology
  • Organ Specificity / physiology
  • Protein Binding / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Cytoplasmic and Nuclear / antagonists & inhibitors*
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Time Factors

Substances

  • Adap1 protein, rat
  • Adaptor Proteins, Signal Transducing
  • Adaptor Proteins, Vesicular Transport
  • Calcium Channels
  • Carrier Proteins
  • GTPase-Activating Proteins
  • Inositol 1,4,5-Trisphosphate Receptors
  • Inositol Phosphates
  • Nerve Tissue Proteins
  • Receptors, Cytoplasmic and Nuclear
  • inositol-1,3,4,5-tetrakisphosphate receptor