Abstract
The protein p42(IP4), expressed mainly in brain, specifically recognizes two second messenger molecules, Ins(1,3,4,5)P(4) (IP(4)), a water soluble metabolite of IP(3) and the lipid PtdIns(3,4,5)P(3) (PIP(3)), the product of the growth factor-activated enzyme PI-3-kinase. Here, we studied whether there is short-term regulation of the expression level of p42(IP4) in limbic brain areas following acoustic and electric stimulation. The stimuli down-regulated the mRNA and protein levels within 2h in amygdala, hypothalamus and cingulate/retrospenial cortex. p42(IP4) mRNA decreased by about 50% for about 24h, but recovered to control values after 72 h. The present results are the first indication of a specific role of p42(IP4) in the short-term regulation of a behavioral response. They indicate that p42(IP4), an adapter protein in PIP(3)-dependent cellular signaling, may play an important role in the signal transduction pathways regulating plasticity in neuronal cells.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Acoustic Stimulation / methods*
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport / antagonists & inhibitors*
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Adaptor Proteins, Vesicular Transport / metabolism
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Animals
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Brain / metabolism*
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Calcium Channels / metabolism
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Carrier Proteins / antagonists & inhibitors*
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Carrier Proteins / metabolism
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Down-Regulation / physiology*
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Electric Stimulation / methods
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GTPase-Activating Proteins
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Inositol 1,4,5-Trisphosphate Receptors
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Inositol Phosphates / metabolism*
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Male
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Nerve Tissue Proteins / antagonists & inhibitors*
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Nerve Tissue Proteins / metabolism
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Neuronal Plasticity / physiology
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Organ Specificity / physiology
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Protein Binding / physiology
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Rats
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Rats, Sprague-Dawley
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Receptors, Cytoplasmic and Nuclear / antagonists & inhibitors*
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Receptors, Cytoplasmic and Nuclear / metabolism
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Time Factors
Substances
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Adap1 protein, rat
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Adaptor Proteins, Signal Transducing
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Adaptor Proteins, Vesicular Transport
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Calcium Channels
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Carrier Proteins
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GTPase-Activating Proteins
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Inositol 1,4,5-Trisphosphate Receptors
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Inositol Phosphates
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Nerve Tissue Proteins
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Receptors, Cytoplasmic and Nuclear
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inositol-1,3,4,5-tetrakisphosphate receptor