Vasoconstrictor responses to 5-hydroxytryptamine in the autoperfused hindquarters of spontaneously hypertensive rats

Pharmacology. 2004 Jun;71(2):66-72. doi: 10.1159/000076942.

Abstract

In this work we studied the responses and receptors involved in the effects of intra-arterial 5-hydroxytryptamine (5-HT) in the in situ autoperfused hindquarters of spontaneously hypertensive rats (SHR). Intra-arterial administration of the highest doses (50-1,000 ng/kg) produced a vasoconstrictor effect that was inhibited by ritanserin (a selective 5-HT2 receptor antagonist), SB 206553 (a selective 5-HT(2B/2C) receptor antagonist) and spiperone (a nonspecific 5-HT(1/2A) receptor antagonist), and was mimicked by alpha-methyl-5-HT (a selective 5-HT2 receptor agonist) and m-CPP (a selective 5-HT2C receptor agonist), but not by the intra-arterial administration of BW 723C86, a selective 5HT2B receptor agonist. SB 206553 and spiperone inhibited alpha-methyl-5HT-induced vasoconstriction in the hindquarters of SHR. Our data suggest that the vasoconstrictor response induced by 5-HT in the autoperfused hindquarters of SHR is mainly mediated by the activation of 5-HT2A and 5-HT2C receptors.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Dose-Response Relationship, Drug
  • Hindlimb / blood supply
  • Hindlimb / drug effects*
  • Hindlimb / physiology
  • Indoles / pharmacology
  • Injections, Intra-Arterial
  • Injections, Intravenous
  • Male
  • Perfusion*
  • Piperazines / pharmacology
  • Prazosin / pharmacology
  • Pyridines / pharmacology
  • Rats
  • Rats, Inbred SHR
  • Rats, Wistar
  • Receptor, Serotonin, 5-HT1A / administration & dosage
  • Receptor, Serotonin, 5-HT2A / administration & dosage
  • Receptor, Serotonin, 5-HT2A / physiology
  • Receptor, Serotonin, 5-HT2B / administration & dosage
  • Receptor, Serotonin, 5-HT2C / administration & dosage
  • Receptor, Serotonin, 5-HT2C / physiology
  • Receptors, Serotonin, 5-HT2 / administration & dosage
  • Regional Blood Flow / drug effects
  • Regional Blood Flow / physiology
  • Ritanserin / pharmacology
  • Serotonin / administration & dosage
  • Serotonin / analogs & derivatives*
  • Serotonin / pharmacokinetics*
  • Serotonin / pharmacology
  • Serotonin / physiology
  • Serotonin 5-HT1 Receptor Antagonists
  • Serotonin 5-HT2 Receptor Agonists
  • Serotonin 5-HT2 Receptor Antagonists
  • Serotonin Receptor Agonists / administration & dosage
  • Serotonin Receptor Agonists / pharmacokinetics
  • Spiperone / pharmacology
  • Thiophenes / pharmacology
  • Vasoconstriction / drug effects*
  • Vasoconstriction / physiology*

Substances

  • 1-(5-(2-thenyloxy)-1H-indol-3-yl)propan-2-amine
  • Indoles
  • Piperazines
  • Pyridines
  • Receptor, Serotonin, 5-HT2A
  • Receptor, Serotonin, 5-HT2B
  • Receptor, Serotonin, 5-HT2C
  • Receptors, Serotonin, 5-HT2
  • Serotonin 5-HT1 Receptor Antagonists
  • Serotonin 5-HT2 Receptor Agonists
  • Serotonin 5-HT2 Receptor Antagonists
  • Serotonin Receptor Agonists
  • Thiophenes
  • Receptor, Serotonin, 5-HT1A
  • Ritanserin
  • alpha-methylserotonin
  • Serotonin
  • Spiperone
  • SB 206553
  • 1-(3-chlorophenyl)piperazine
  • Prazosin