Abstract
In this work we studied the responses and receptors involved in the effects of intra-arterial 5-hydroxytryptamine (5-HT) in the in situ autoperfused hindquarters of spontaneously hypertensive rats (SHR). Intra-arterial administration of the highest doses (50-1,000 ng/kg) produced a vasoconstrictor effect that was inhibited by ritanserin (a selective 5-HT2 receptor antagonist), SB 206553 (a selective 5-HT(2B/2C) receptor antagonist) and spiperone (a nonspecific 5-HT(1/2A) receptor antagonist), and was mimicked by alpha-methyl-5-HT (a selective 5-HT2 receptor agonist) and m-CPP (a selective 5-HT2C receptor agonist), but not by the intra-arterial administration of BW 723C86, a selective 5HT2B receptor agonist. SB 206553 and spiperone inhibited alpha-methyl-5HT-induced vasoconstriction in the hindquarters of SHR. Our data suggest that the vasoconstrictor response induced by 5-HT in the autoperfused hindquarters of SHR is mainly mediated by the activation of 5-HT2A and 5-HT2C receptors.
Copyright 2004 S. Karger AG, Basel
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Dose-Response Relationship, Drug
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Hindlimb / blood supply
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Hindlimb / drug effects*
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Hindlimb / physiology
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Indoles / pharmacology
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Injections, Intra-Arterial
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Injections, Intravenous
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Male
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Perfusion*
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Piperazines / pharmacology
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Prazosin / pharmacology
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Pyridines / pharmacology
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Rats
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Rats, Inbred SHR
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Rats, Wistar
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Receptor, Serotonin, 5-HT1A / administration & dosage
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Receptor, Serotonin, 5-HT2A / administration & dosage
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Receptor, Serotonin, 5-HT2A / physiology
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Receptor, Serotonin, 5-HT2B / administration & dosage
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Receptor, Serotonin, 5-HT2C / administration & dosage
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Receptor, Serotonin, 5-HT2C / physiology
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Receptors, Serotonin, 5-HT2 / administration & dosage
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Regional Blood Flow / drug effects
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Regional Blood Flow / physiology
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Ritanserin / pharmacology
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Serotonin / administration & dosage
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Serotonin / analogs & derivatives*
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Serotonin / pharmacokinetics*
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Serotonin / pharmacology
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Serotonin / physiology
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Serotonin 5-HT1 Receptor Antagonists
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Serotonin 5-HT2 Receptor Agonists
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Serotonin 5-HT2 Receptor Antagonists
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Serotonin Receptor Agonists / administration & dosage
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Serotonin Receptor Agonists / pharmacokinetics
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Spiperone / pharmacology
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Thiophenes / pharmacology
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Vasoconstriction / drug effects*
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Vasoconstriction / physiology*
Substances
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1-(5-(2-thenyloxy)-1H-indol-3-yl)propan-2-amine
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Indoles
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Piperazines
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Pyridines
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Receptor, Serotonin, 5-HT2A
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Receptor, Serotonin, 5-HT2B
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Receptor, Serotonin, 5-HT2C
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Receptors, Serotonin, 5-HT2
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Serotonin 5-HT1 Receptor Antagonists
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Serotonin 5-HT2 Receptor Agonists
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Serotonin 5-HT2 Receptor Antagonists
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Serotonin Receptor Agonists
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Thiophenes
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Receptor, Serotonin, 5-HT1A
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Ritanserin
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alpha-methylserotonin
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Serotonin
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Spiperone
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SB 206553
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1-(3-chlorophenyl)piperazine
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Prazosin